RNF19B sirna

RNF19B siRNA (Human)

Cat. Num. AAA8214820

• Gene Names: RNF19B; NKLAM; IBRDC3
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RNF19B; RNF19B siRNA (Human); IBRDC3; NKLAM; E3 ubiquitin-protein ligase RNF19B; IBR domain-containing protein 3; Natural killer lytic-associated molecule; RING finger protein 19B; RNF19B sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: RNF19B siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 587

• Applicable Applications for RNF19B sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human RNF19B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RNF19B sirna

siRNA to inhibit RNF19B expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 188219579
• NCBI GeneID: 127544
• NCBI Accession #: NP_001120833.1
• NCBI GenBank Nucleotide #: NM_001127361.2
• UniProt Accession #: Q6ZMZ0; Q0VG77; Q5TH44; Q5TH45; Q6P6A4; Q8N2S8; Q8WUF3; B7ZLB2; E9PAW6; G3XA82
• Molecular Weight: 77,854 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase RNF19B isoform b
• NCBI Official Synonym Full Names: ring finger protein 19B
• NCBI Official Symbol: RNF19B
• NCBI Official Synonym Symbols: NKLAM; IBRDC3
• NCBI Protein Information: E3 ubiquitin-protein ligase RNF19B
• UniProt Protein Name: E3 ubiquitin-protein ligase RNF19B
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: RNF19B
• UniProt Synonym Gene Names: IBRDC3; NKLAM
• UniProt Entry Name: RN19B_HUMAN

NCBI Description

This gene encodes a multi-pass membrane protein containing two RING-type and one IBR-type zinc finger motifs. The encoded protin is an E3 ubiquitin-protein ligase that plays a role in the cytotoxic effects of natural killer (NK) cells. Alternative splicing results in multiple transcript variants. There are pseudogenes for this gene on chromosomes X and Y in a possible pseudoautosomal region. [provided by RefSeq, Jul 2014]

Uniprot Description

RNF19B: E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates, such as UCKL1. Involved in the cytolytic activity of natural killer cells and cytotoxic T-cells. Belongs to the RBR family. RNF19 subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Ubiquitin ligase; Ligase; Ubiquitin conjugating system; EC 6.3.2.-; Membrane protein, multi-pass; EC 6.3.2.19

Chromosomal Location of Human Ortholog: 1p35.1

Cellular Component: cytoplasm; integral to membrane

Molecular Function: protein binding; zinc ion binding; ligase activity

Biological Process: natural killer cell mediated cytotoxicity; protein ubiquitination