NSD1 sirna

NSD1 siRNA (Human)

Cat. Num. AAA8214749

• Gene Names: NSD1; STO; KMT3B; SOTOS; ARA267; SOTOS1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: NSD1; NSD1 siRNA (Human); ARA267; KMT3B; Histone-lysine N-methyltransferase. H3 lysine-36 and H4 lysine-20 specific; Androgen receptor coactivator 267 kDa protein; Androgen receptor-associated protein of 267 kDa; H3-K36-HMTase; H4-K20-HMTase; Lysine N-methyltransferase 3B; Nuclear receptor-binding SET domain-containing protein 1; NR-binding SET domain-containing protein; NSD1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: NSD1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 2696

• Applicable Applications for NSD1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human NSD1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for NSD1 sirna

siRNA to inhibit NSD1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 19923586
• NCBI GeneID: 64324
• NCBI Accession #: NP_071900.2
• NCBI GenBank Nucleotide #: NM_022455.4
• UniProt Accession #: Q96L73; Q96PD8; Q96RN7
• Molecular Weight: 284,264 Da
• NCBI Official Full Name: histone-lysine N-methyltransferase, H3 lysine-36 and H4 lysine-20 specific isoform b
• NCBI Official Synonym Full Names: nuclear receptor binding SET domain protein 1
• NCBI Official Symbol: NSD1
• NCBI Official Synonym Symbols: STO; KMT3B; SOTOS; ARA267; SOTOS1
• NCBI Protein Information: histone-lysine N-methyltransferase, H3 lysine-36 and H4 lysine-20 specific
• UniProt Protein Name: Histone-lysine N-methyltransferase, H3 lysine-36 and H4 lysine-20 specific
• Protein Family: Histone-lysine N-methyltransferase
• UniProt Gene Name: NSD1
• UniProt Synonym Gene Names: ARA267; KMT3B; NR-binding SET domain-containing protein
• UniProt Entry Name: NSD1_HUMAN

NCBI Description

This gene encodes a protein containing a SET domain, 2 LXXLL motifs, 3 nuclear translocation signals (NLSs), 4 plant homeodomain (PHD) finger regions, and a proline-rich region. The encoded protein enhances androgen receptor (AR) transactivation, and this enhancement can be increased further in the presence of other androgen receptor associated coregulators. This protein may act as a nucleus-localized, basic transcriptional factor and also as a bifunctional transcriptional regulator. Mutations of this gene have been associated with Sotos syndrome and Weaver syndrome. One version of childhood acute myeloid leukemia is the result of a cryptic translocation with the breakpoints occurring within nuclear receptor-binding Su-var, enhancer of zeste, and trithorax domain protein 1 on chromosome 5 and nucleoporin, 98-kd on chromosome 11. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

NSD1: Histone methyltransferase. Preferentially methylates 'Lys-36' of histone H3 and 'Lys-20' of histone H4 (in vitro). Transcriptional intermediary factor capable of both negatively or positively influencing transcription, depending on the cellular context. Defects in NSD1 are the cause of Sotos syndrome type 1 (SOTOS1); also known as cerebral gigantism. It is a disorder characterized by excessively rapid growth, acromegalic features, and a nonprogressive cerebral disorder with mental retardation. High-arched palate and prominent jaw are noted in several patients. Most cases of Sotos syndrome are sporadic and may represent new dominant mutation. Defects in NSD1 are the cause of Weaver syndrome type 1 (WVS1). A syndrome of accelerated growth and osseous maturation, unusual craniofacial appearance, hoarse and low- pitched cry, and hypertonia with camptodactyly. Distinguishing features of Weaver syndrome include broad forehead and face, ocular hypertelorism, prominent wide philtrum, micrognathia, deep horizontal chin groove, and deep-set nails. In addition, carpal bone development is advanced over the rest of the hand. Defects in NSD1 are a cause of Beckwith-Wiedemann syndrome (BWS). BWS is a genetically heterogeneous disorder characterized by anterior abdominal wall defects including exomphalos (omphalocele), pre- and postnatal overgrowth, and macroglossia. Additional less frequent complications include specific developmental defects and a predisposition to embryonal tumors. A chromosomal aberration involving NSD1 is found in childhood acute myeloid leukemia. Translocation t(5;11)(q35;p15.5) with NUP98. A chromosomal aberration involving NSD1 is found in an adult form of myelodysplastic syndrome (MDS). Insertion of NUP98 into NSD1 generates a NUP98-NSD1 fusion product. Belongs to the histone-lysine methyltransferase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Oncoprotein; Amino Acid Metabolism - lysine degradation; Methyltransferase; Methyltransferase, protein lysine; EC 2.1.1.43; Transcription, coactivator/corepressor; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 5q35

Cellular Component: nucleoplasm; nucleolus; chromosome; nucleus

Molecular Function: retinoid X receptor binding; androgen receptor binding; zinc ion binding; retinoic acid receptor binding; histone lysine N-methyltransferase activity (H4-K20 specific); transcription cofactor activity; estrogen receptor binding; thyroid hormone receptor binding; chromatin binding; transcription corepressor activity; histone lysine N-methyltransferase activity (H3-K36 specific)

Biological Process: histone methylation; establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; histone H3-K36 methylation; negative regulation of transcription from RNA polymerase II promoter; gastrulation with mouth forming second

Disease: Sotos Syndrome 1; Beckwith-wiedemann Syndrome