PHF2 sirna

PHF2 siRNA (Mouse)

Cat. Num. AAA8214328

• Gene Names: Phf2; GRC5
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PHF2; PHF2 siRNA (Mouse); KIAA0662; Lysine-specific demethylase PHF2; GRC5; PHD finger protein 2; PHF2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: PHF2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1096

• Applicable Applications for PHF2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse PHF2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PHF2 sirna

siRNA to inhibit PHF2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 31543476
• NCBI GeneID: 18676
• NCBI Accession #: NP_035208.2
• NCBI GenBank Nucleotide #: NM_011078.3
• UniProt Accession #: Q9WTU0; Q6A023; Q80WA8
• Molecular Weight: 120,814 Da
• NCBI Official Full Name: lysine-specific demethylase PHF2
• NCBI Official Synonym Full Names: PHD finger protein 2
• NCBI Official Symbol: Phf2
• NCBI Official Synonym Symbols: GRC5
• NCBI Protein Information: lysine-specific demethylase PHF2
• UniProt Protein Name: Lysine-specific demethylase PHF2
• Protein Family: Lysine-specific demethylase
• UniProt Gene Name: Phf2
• UniProt Synonym Gene Names: Kiaa0662
• UniProt Entry Name: PHF2_MOUSE

Uniprot Description

PHF2: Lysine demethylase that demethylates both histones and non-histone proteins. Enzymatically inactive by itself, and becomes active following phosphorylation by PKA: forms a complex with ARID5B and mediates demethylation of methylated ARID5B. Demethylation of ARID5B leads to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver. PHF2 is recruited to trimethylated 'Lys-4' of histone H3 (H3K4me3) at rDNA promoters and promotes expression of rDNA. Component of the PHF2-ARID5B complex, at least composed of PHF2 and ARID5B. Interacts with HNF4A and NR1H4. Widely expressed, including in liver. Enzymatically inactive by itself, and become active following phosphorylation by PKA. Belongs to the JHDM1 histone demethylase family. JHDM1D subfamily.

Protein type: EC 1.14.11.-; Nucleolus

Cellular Component: kinetochore; cytoplasm; nucleolus; chromosome; nucleus; chromosome, pericentric region

Molecular Function: procollagen-proline dioxygenase activity; 2,4-dichlorophenoxyacetate alpha-ketoglutarate dioxygenase activity; sulfonate dioxygenase activity; dioxygenase activity; zinc ion binding; metal ion binding; iron ion binding; transcription coactivator activity; DNA-N1-methyladenine dioxygenase activity; oxidoreductase activity; histone demethylase activity (H3-K9 specific); methylated histone residue binding

Biological Process: histone H3-K9 demethylation; transcription, DNA-dependent; regulation of transcription, DNA-dependent; chromatin modification; protein amino acid demethylation