DFNB59 sirna

DFNB59 siRNA (Human)

Cat. Num. AAA8214229

• Gene Names: DFNB59; PJVK
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DFNB59; DFNB59 siRNA (Human); PJVK; Pejvakin; Autosomal recessive deafness type 59 protein; DFNB59 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: DFNB59 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 352

• Applicable Applications for DFNB59 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human DFNB59 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DFNB59 sirna

siRNA to inhibit DFNB59 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 111607457
• NCBI GeneID: 494513
• NCBI Accession #: NP_001036167.1
• NCBI GenBank Nucleotide #: NM_001042702.3
• UniProt Accession #: Q0ZLH3; A0PK14; B9EJE2
• Molecular Weight: 39,913 Da
• NCBI Official Full Name: pejvakin
• NCBI Official Synonym Full Names: deafness, autosomal recessive 59
• NCBI Official Symbol: DFNB59
• NCBI Official Synonym Symbols: PJVK
• NCBI Protein Information: pejvakin
• UniProt Protein Name: Pejvakin
• UniProt Gene Name: DFNB59
• UniProt Synonym Gene Names: PJVK
• UniProt Entry Name: PJVK_HUMAN

NCBI Description

The protein encoded by this gene is a member of the gasdermin family, a family which is found only in vertebrates. The encoded protein is required for the proper function of auditory pathway neurons. Defects in this gene are a cause of non-syndromic sensorineural deafness autosomal recessive type 59 (DFNB59). [provided by RefSeq, Dec 2008]

Uniprot Description

DFNB59: Essential in the activity of auditory pathway neurons. Defects in PJVK are the cause of deafness autosomal recessive type 59 (DFNB59). DFNB59 is a form of sensorineural hearing impairment with absent or severely abnormal auditory brainstem response but normal otoacoustic emissions (auditory neuropathy or auditory dys-synchrony). Auditory neuropathies result from a lesion in the area including the inner hair cells, connections between the inner hair cells and the cochlear branch of the auditory nerve, the auditory nerve itself and auditory pathways of the brainstem. Belongs to the gasdermin family.

Chromosomal Location of Human Ortholog: 2q31.2

Cellular Component: cell soma

Biological Process: sensory perception of sound

Disease: Deafness, Autosomal Recessive 59