APOA4 sirna

APOA4 siRNA (Rat)

Cat. Num. AAA8214164

• Gene Names: Apoa4; apoAIV; Apo-AIV; ApoA-IV
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: APOA4; APOA4 siRNA (Rat); Apolipoprotein A-IV; Apo-AIV; ApoA-IV; Apolipoprotein A4; APOA4 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: APOA4 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 391

• Applicable Applications for APOA4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat APOA4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for APOA4 sirna

siRNA to inhibit APOA4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 8392909
• NCBI GeneID: 25080
• NCBI Accession #: NP_036869.1
• NCBI GenBank Nucleotide #: NM_012737.1
• UniProt Accession #: P02651; Q5BK92
• Molecular Weight: 44,456 Da
• NCBI Official Full Name: apolipoprotein A-IV
• NCBI Official Synonym Full Names: apolipoprotein A-IV
• NCBI Official Symbol: Apoa4
• NCBI Official Synonym Symbols: apoAIV; Apo-AIV; ApoA-IV
• NCBI Protein Information: apolipoprotein A-IV
• UniProt Protein Name: Apolipoprotein A-IV
• Protein Family: Apolipoprotein
• UniProt Gene Name: Apoa4
• UniProt Synonym Gene Names: Apo-AIV; ApoA-IV
• UniProt Entry Name: APOA4_RAT

NCBI Description

may play a role in lipid transport [RGD, Feb 2006]

Uniprot Description

APOA4: May have a role in chylomicrons and VLDL secretion and catabolism. Required for efficient activation of lipoprotein lipase by ApoC-II; potent activator of LCAT. Apoa-IV is a major component of HDL and chylomicrons. Synthesized primarily in the intestine and secreted in plasma. Belongs to the apolipoprotein A1/A4/E family.

Protein type: Secreted, signal peptide; Lipid-binding; Cell adhesion; Secreted; Endoplasmic reticulum

Cellular Component: extracellular space; chylomicron; cell surface

Molecular Function: antioxidant activity; copper ion binding; protein homodimerization activity; cholesterol transporter activity; cholesterol binding; phosphatidylcholine binding; lipid binding

Biological Process: response to food; multicellular organismal lipid catabolic process; positive regulation of fatty acid biosynthetic process; cholesterol metabolic process; removal of superoxide radicals; lipid homeostasis; protein-lipid complex assembly; cholesterol efflux; lipoprotein metabolic process; lipid transport; cholesterol biosynthetic process; axon regeneration in the peripheral nervous system; phosphatidylcholine metabolic process; cholesterol homeostasis; leukocyte adhesion; reverse cholesterol transport; regulation of cholesterol absorption; innate immune response in mucosa; phospholipid efflux; response to lipid hydroperoxide; hydrogen peroxide catabolic process; response to triglyceride; positive regulation of lipoprotein lipase activity; regulation of cholesterol transport