RAET1G sirna

RAET1G siRNA (Human)

Cat. Num. AAA8213896

• Gene Names: RAET1G; ULBP5
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RAET1G; RAET1G siRNA (Human); Retinoic acid early transcript 1G protein; RAET1G sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: RAET1G siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 334

• Applicable Applications for RAET1G sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human RAET1G gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RAET1G sirna

siRNA to inhibit RAET1G expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 130484306
• NCBI GeneID: 353091
• NCBI Accession #: NP_001001788.2
• NCBI GenBank Nucleotide #: NM_001001788.3
• UniProt Accession #: Q6H3X3; Q6H3X2
• Molecular Weight: 23,884 Da
• NCBI Official Full Name: retinoic acid early transcript 1G protein preproprotein
• NCBI Official Synonym Full Names: retinoic acid early transcript 1G
• NCBI Official Symbol: RAET1G
• NCBI Official Synonym Symbols: ULBP5
• NCBI Protein Information: retinoic acid early transcript 1G protein
• UniProt Protein Name: Retinoic acid early transcript 1G protein
• Protein Family: Retinoic acid early transcript 1G protein
• UniProt Gene Name: RAET1G
• UniProt Synonym Gene Names: ULBP5
• UniProt Entry Name: RET1G_HUMAN

NCBI Description

This gene encodes a member of the major histocompatibility complex (MHC) class I family of proteins. Although the encoded protein includes C-terminal transmembrane and cytoplasmic domains, proteolytic processing results in the removal of these domains and subsequent tethering to the plasma membrane by a glycosylphosphatidylinositol (GPI)-anchor. The encoded protein is one of several related ligands of the natural killer group 2, member D (NKG2D) receptor, which functions as an activating receptor in innate and adaptive immunity. This gene is present in a gene cluster on chromosome 6. [provided by RefSeq, Jul 2015]

Uniprot Description

RAET1G: Acts as a ligand for the NKG2D receptor and mediates NK cell cytotoxicity via the receptor. Belongs to the MHC class I family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 6q24.1-q25.1

Cellular Component: endoplasmic reticulum; extracellular region; integral to membrane; plasma membrane

Molecular Function: peptide antigen binding; natural killer cell lectin-like receptor binding

Biological Process: antigen processing and presentation of peptide antigen via MHC class I; natural killer cell mediated cytotoxicity