MLC1 sirna

MLC1 siRNA (Mouse)

Cat. Num. AAA8213839

• Gene Names: Mlc1; VL; LVM; MLC; WKL1; AW048630; BB074274; mKIAA0027; Kiaa0027-hp
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MLC1; MLC1 siRNA (Mouse); Membrane protein MLC1; MLC1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: MLC1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 382

• Applicable Applications for MLC1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse MLC1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MLC1 sirna

siRNA to inhibit MLC1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 18875410
• NCBI GeneID: 170790
• NCBI Accession #: NP_573504.1
• NCBI GenBank Nucleotide #: NM_133241.2
• UniProt Accession #: Q8VHK5
• Molecular Weight: 41,596 Da
• NCBI Official Full Name: membrane protein MLC1
• NCBI Official Synonym Full Names: megalencephalic leukoencephalopathy with subcortical cysts 1 homolog (human)
• NCBI Official Symbol: Mlc1
• NCBI Official Synonym Symbols: VL; LVM; MLC; WKL1; AW048630; BB074274; mKIAA0027; Kiaa0027-hp
• NCBI Protein Information: membrane protein MLC1
• UniProt Protein Name: Membrane protein MLC1
• Protein Family: Myosin
• UniProt Gene Name: Mlc1
• UniProt Entry Name: MLC1_MOUSE

Uniprot Description

MLC1: Regulates the response of astrocytes to hypo-osmosis by promoting calcium influx. Defects in MLC1 are a cause of leukoencephalopathy megalencephalic with subcortical cysts type 1 (MLC1). MLC1 is a syndrome of cerebral leukoencephalopathy and megalencephaly characterized by ataxia, spasticity, seizures, delay in motor development and mild mental retardation. The brain appears swollen on magnetic resonance imaging, with diffuse white- matter abnormalities and the invariable presence of subcortical cysts in frontal and temporal lobes.

Protein type: Membrane protein, integral; Cell surface; Transporter; Membrane protein, multi-pass

Cellular Component: lysosome; basolateral plasma membrane; endoplasmic reticulum; early endosome; integral to membrane; intercellular junction; caveola; lipid raft; recycling endosome; membrane; perinuclear region of cytoplasm; apical plasma membrane; cytoplasm; plasma membrane; cytoplasmic vesicle; endosome

Molecular Function: protein binding; ion channel activity; protein complex binding; protein transporter activity

Biological Process: vesicle-mediated transport; protein transport; transport; positive regulation of intracellular transport; ion transport; protein oligomerization; regulation of response to osmotic stress