PPP2CA sirna

PPP2CA siRNA (Rat)

Cat. Num. AAA8213526

• Gene Names: Ppp2ca; Pp2a1
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PPP2CA; PPP2CA siRNA (Rat); Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform; PP2A-alpha; PPP2CA sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: PPP2CA siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 309

• Applicable Applications for PPP2CA sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat PPP2CA gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PPP2CA sirna

siRNA to inhibit PPP2CA expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 8394018
• NCBI GeneID: 24672
• NCBI Accession #: NP_058735.1
• NCBI GenBank Nucleotide #: NM_017039.2
• UniProt Accession #: P63331; O88591; P13353
• Molecular Weight: 35,608 Da
• NCBI Official Full Name: serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
• NCBI Official Synonym Full Names: protein phosphatase 2, catalytic subunit, alpha isozyme
• NCBI Official Symbol: Ppp2ca
• NCBI Official Synonym Symbols: Pp2a1
• NCBI Protein Information: serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
• UniProt Protein Name: Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
• Protein Family: Serine/threonine-protein phosphatase
• UniProt Gene Name: Ppp2ca
• UniProt Synonym Gene Names: PP2A-alpha
• UniProt Entry Name: PP2AA_RAT

NCBI Description

catalyzes the removal of serine- and threonine- bound phosphate groups [RGD, Feb 2006]

Uniprot Description

PPP2CA: ubiquitous cytosolic Ser/Thr protein phosphatase, catalytic subunit. The holoenzyme consists of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B'/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules.

Protein type: Protein phosphatase, Ser/Thr (non-receptor); Motility/polarity/chemotaxis; EC 3.1.3.16

Cellular Component: spindle pole; neuron projection; protein phosphatase type 2A complex; cytoplasm; postsynaptic density; plasma membrane; terminal button; nucleus; cytosol; chromosome, pericentric region

Molecular Function: protein C-terminus binding; protein dimerization activity; protein kinase B binding; protein domain specific binding; protein phosphatase 2A binding; metal ion binding; protein serine/threonine phosphatase activity; protein kinase binding; protein phosphatase binding; protein binding; enzyme binding; protein heterodimerization activity; GABA receptor binding; protein complex binding; tau protein binding

Biological Process: regulation of protein amino acid phosphorylation; positive regulation of apoptosis; regulation of protein amino acid autophosphorylation; heart development; positive regulation of protein phosphatase type 2A activity; positive regulation of caspase activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription factor import into nucleus; protein amino acid dephosphorylation; positive regulation of protein amino acid dephosphorylation; meiotic cell cycle; cellular response to insulin stimulus; negative regulation of protein amino acid phosphorylation; regulation of protein catabolic process; mesoderm development; regulation of receptor activity