Testing Data
(Figure 1: Immunocytochemistry on Cadherin 11 transfected PC3 cells)
Mouse anti-Human, Rat OB-Cadherin / Cadherin-11 Monoclonal Antibody | anti-CDH11 antibody
Mouse anti OB-Cadherin / Cadherin-11
Gene Names
CDH11; OB; CAD11; CDHOB; OSF-4
Reactivity
Human, Rat
Applications
Immunoblot, Western Blot, Immunocytochemistry, Immunohistochemistry, Western Blot
Synonyms
OB-Cadherin / Cadherin-11; Monoclonal Antibody; Mouse anti OB-Cadherin / Cadherin-11; anti-CDH11 antibody
Host
Mouse
Reactivity
Human, Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
16A
Specificity
16A recognizes the extracellular domain of cadherin-11.
Form/Format
Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
Applicable Applications for anti-CDH11 antibody
Immunoblotting (IB), Immunocytochemistry (ICC), Immunohistochemistry-Frozen (IHC-Fr), Western Blotting (WB)
Application Notes
16A is suitable for immunoblotting, immunocytochemistry and immunohistochemistry on frozen sections when using a PBS buffer containing 0.1 mM CaCl2 and 0.1 mM MgCl2. Optimal antibody dilution should be determined by titration; recommended range is 1:25 – 1:50 for immunohistochemistry with avidin- biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:25 – 1:250 for immunoblotting applications.
Product Type
Primary Antibodies
Host Note
16A is a Mouse monoclonal IgG1 antibody obtained by fusion of SP2/0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with affinity purified extracellular domain of Human cadherin-11-GST fusion protein.
Caution
This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water.
Preparation and Storage
The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.
Testing Data
(Figure 1: Immunocytochemistry on Cadherin 11 transfected PC3 cells)
Testing Data
(Figure 1: Immunocytochemistry on Cadherin 11 transfected PC3 cells)
Testing Data
(Figure 2: Western blot on a lysate of Cadherin 11 transfected PC3 cells)
Testing Data
(Figure 2: Western blot on a lysate of Cadherin 11 transfected PC3 cells)
Related Product Information for anti-CDH11 antibody
Cadherins constitute a family of transmembrane glycoproteins involved in Ca2+-dependent cell-cell interactions. The members of this family are differentially expressed in various tissues. They function in the maintenance of tissue integrity and morphogenesis. The cadherins generally contain five extracellular repeats, a transmembrane domain and a cytoplasmic tail that binds to the Catenin family of cytoskeletal anchoring proteins which also function as signal transducers. The extracellular domains are responsible for the specificity of homophilic interactions between cells expressing the same cadherin. Cadherins are divided into type I and type II subgroups. Type I cadherins include epithelial cadherin (E-cadherin, cadherin-1 or uvomorulin), neural cadherin (N-cadherin or cadherin-2), placental cadherin (P-cadherin or cadherin-3) and retinal cadherin (R-cadherin or cadherin- 4). Kidney cadherin (K-cadherin or cadherin-6) and osteoblast cadherin (OB-cadherin or cadherin-11) are type II cadherins. The progression of carcinomas is associated with the loss of epithelial morphology and a concomitant acquisition of a more mesenchymal phenotype, which is thought to contribute to the invasive and/or metastatic behavior. A putative role for cadherin-11 in these late stages of tumor progression is based on the fact that migRation of mesenchymal cells is facilitated when cadherin-11 is highly expressed.
Product Categories/Family for anti-CDH11 antibody
References
Bussemakers, M. J., Van Bokhoven, A., Tomita, K., Jansen, C. F., and Schalken, J. A. (2000). Complex cadherin expression in Human prostate cancer cells, Int J Cancer 85, 446-50. Tomita, K., van Bokhoven, A., van Leenders, G. J., Ruijter, E. T., Jansen, C. F., Bussemakers, M. J., and Schalken, J. A. (2000). Cadherin switching in Human prostate cancer progression, Cancer Res 60, 3650-4.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
cadherin-11 preproprotein
NCBI Official Synonym Full Names
cadherin 11, type 2, OB-cadherin (osteoblast)
NCBI Official Symbol
CDH11
NCBI Official Synonym Symbols
OB; CAD11; CDHOB; OSF-4
NCBI Protein Information
cadherin-11; OTTHUMP00000174770; OTTHUMP00000174771
UniProt Protein Name
Cadherin-11
Protein Family
UniProt Gene Name
CDH11
UniProt Entry Name
CAD11_HUMAN
NCBI Description
This gene encodes a type II classical cadherin from the cadherin superfamily, integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. Expression of this particular cadherin in osteoblastic cell lines, and its upregulation during differentiation, suggests a specific function in bone development and maintenance. [provided by RefSeq]
Uniprot Description
CDH11: Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Interacts with PCDH8. Expressed mainly in brain but also found in other tissues. Expressed in neuroblasts. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Cell adhesion
Chromosomal Location of Human Ortholog: 16q21
Cellular Component: cytoplasm; integral to membrane; plasma membrane
Molecular Function: calcium ion binding
Biological Process: intercellular junction assembly and maintenance; ossification; corticospinal tract morphogenesis; cell adhesion; homophilic cell adhesion; skeletal development
Research Articles on CDH11
Similar Products
Product Notes
The CDH11 cdh11 (Catalog #AAA570041) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti OB-Cadherin / Cadherin-11 reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's OB-Cadherin / Cadherin-11 can be used in a range of immunoassay formats including, but not limited to, Immunoblotting (IB), Immunocytochemistry (ICC), Immunohistochemistry-Frozen (IHC-Fr), Western Blotting (WB). 16A is suitable for immunoblotting, immunocytochemistry and immunohistochemistry on frozen sections when using a PBS buffer containing 0.1 mM CaCl2 and 0.1 mM MgCl2. Optimal antibody dilution should be determined by titration; recommended range is 1:25 – 1:50 for immunohistochemistry with avidin- biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:25 – 1:250 for immunoblotting applications. Researchers should empirically determine the suitability of the CDH11 cdh11 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "OB-Cadherin / Cadherin-11, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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