PIGA sirna

PIGA siRNA (Mouse)

Cat. Num. AAA8213113

• Gene Names: Piga; Pig-a; AI194334
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PIGA; PIGA siRNA (Mouse); N-acetylglucosaminyl-phosphatidylinositol biosynthetic protein; GlcNAc-PI synthesis protein; Phosphatidylinositol-glycan biosynthesis class A protein; PIG-A; PIGA sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: PIGA siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 485

• Applicable Applications for PIGA sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse PIGA gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PIGA sirna

siRNA to inhibit PIGA expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 126273666
• NCBI GeneID: 18700
• NCBI Accession #: NP_035211.2
• NCBI GenBank Nucleotide #: NM_011081.2
• UniProt Accession #: Q64323; Q6LD71; Q8CCQ6
• Molecular Weight: 54,469 Da
• NCBI Official Full Name: N-acetylglucosaminyl-phosphatidylinositol biosynthetic protein
• NCBI Official Synonym Full Names: phosphatidylinositol glycan anchor biosynthesis, class A
• NCBI Official Symbol: Piga
• NCBI Official Synonym Symbols: Pig-a; AI194334
• NCBI Protein Information: N-acetylglucosaminyl-phosphatidylinositol biosynthetic protein
• UniProt Protein Name: N-acetylglucosaminyl-phosphatidylinositol biosynthetic protein
• Protein Family: Phosphatidylinositol N-acetylglucosaminyltransferase
• UniProt Gene Name: Piga
• UniProt Synonym Gene Names: PIG-A
• UniProt Entry Name: PIGA_MOUSE

Uniprot Description

PIGA: Necessary for the synthesis of N-acetylglucosaminyl- phosphatidylinositol, the very early intermediate in GPI-anchor biosynthesis. Defects in PIGA are the cause of paroxysmal nocturnal hemoglobinuria (PNH). PNH is a disorder characterized by hemolytic anemia with hemoglobinuria, thromboses in large vessels, and a deficiency in hematopoiesis. Clinical manifestation of red blood cell breakdown with release of hemoglobin into the urine is manifested most prominently by dark-colored urine in the morning. Defects in PIGA are the cause of multiple congenital anomalies-hypotonia-seizures syndrome type 2 (MCAHS2). An X-linked recessive developmental disorder characterized by dysmorphic features, neonatal hypotonia, myoclonic seizures, and variable congenital anomalies involving the central nervous, cardiac, and urinary systems. Most affected individuals die in infancy. Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Glycan Metabolism - glycosylphosphatidylinositol (GPI)-anchor biosynthesis; Transferase; EC 2.4.1.198

Cellular Component: endoplasmic reticulum membrane; glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex; membrane; endoplasmic reticulum; integral to membrane

Molecular Function: transferase activity; transferase activity, transferring glycosyl groups

Biological Process: GPI anchor biosynthetic process; biosynthetic process