DPM3 sirna

DPM3 siRNA (Human)

Cat. Num. AAA8212640

• Gene Names: DPM3; CDG1O
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DPM3; DPM3 siRNA (Human); Dolichol-phosphate mannosyltransferase subunit 3; Dolichol-phosphate mannose synthase subunit 3; DPM synthase subunit 3; Dolichyl-phosphate beta-D-mannosyltransferase subunit 3; Mannose-P-dolichol synthase subunit 3; MPD synthase subunit 3; Prostin-1; DPM3 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: DPM3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 122

• Applicable Applications for DPM3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human DPM3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DPM3 sirna

siRNA to inhibit DPM3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 19424120
• NCBI GeneID: 54344
• NCBI Accession #: NP_061846.2
• NCBI GenBank Nucleotide #: NM_018973.3
• UniProt Accession #: Q9P2X0; Q5SR62; Q5SR63; Q9BXN4; Q9BXN5
• Molecular Weight: 13,277 Da
• NCBI Official Full Name: dolichol-phosphate mannosyltransferase subunit 3 isoform 1
• NCBI Official Synonym Full Names: dolichyl-phosphate mannosyltransferase polypeptide 3
• NCBI Official Symbol: DPM3
• NCBI Official Synonym Symbols: CDG1O
• NCBI Protein Information: dolichol-phosphate mannosyltransferase subunit 3
• UniProt Protein Name: Dolichol-phosphate mannosyltransferase subunit 3
• Protein Family: Dolichol-phosphate mannosyltransferase
• UniProt Gene Name: DPM3
• UniProt Synonym Gene Names: DPM synthase subunit 3; MPD synthase subunit 3
• UniProt Entry Name: DPM3_HUMAN

NCBI Description

Dolichol-phosphate mannose (Dol-P-Man) serves as a donor of mannosyl residues on the lumenal side of the endoplasmic reticulum (ER). Lack of Dol-P-Man results in defective surface expression of GPI-anchored proteins. Dol-P-Man is synthesized from GDP-mannose and dolichol-phosphate on the cytosolic side of the ER by the enzyme dolichyl-phosphate mannosyltransferase. The protein encoded by this gene is a subunit of dolichyl-phosphate mannosyltransferase and acts as a stabilizer subunit of the dolichyl-phosphate mannosyltransferase complex. [provided by RefSeq, Jul 2008]

Uniprot Description

DPM3: Stabilizer subunit of the dolichol-phosphate-mannose synthase complex. Defects in DPM3 are the cause of congenital disorder of glycosylation type 1O (CDG1O). A multisystem disorder caused by a defect in glycoprotein biosynthesis and characterized by under-glycosylated serum glycoproteins. Congenital disorders of glycosylation result in a wide variety of clinical features, such as defects in the nervous system development, psychomotor retardation, dysmorphic features, hypotonia, coagulation disorders, and immunodeficiency. The broad spectrum of features reflects the critical role of N-glycoproteins during embryonic development, differentiation, and maintenance of cell functions. Belongs to the DPM3 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Transferase; Endoplasmic reticulum; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q22

Cellular Component: endoplasmic reticulum membrane; membrane; endoplasmic reticulum; integral to endoplasmic reticulum membrane; mannosyltransferase complex

Molecular Function: protein binding; dolichyl-phosphate beta-D-mannosyltransferase activity

Biological Process: cellular protein metabolic process; protein amino acid mannosylation; protein amino acid O-linked mannosylation; dolichol-linked oligosaccharide biosynthetic process; carbohydrate metabolic process; GPI anchor biosynthetic process; regulation of protein stability; protein amino acid N-linked glycosylation via asparagine; C-terminal protein lipidation; post-translational protein modification; protein amino acid C-linked glycosylation via 2'-alpha-mannosyl-L-tryptophan

Disease: Congenital Disorder Of Glycosylation, Type Io