ARNTL2 sirna

ARNTL2 siRNA (Human)

Cat. Num. AAA8212423

• Gene Names: ARNTL2; CLIF; MOP9; BMAL2; PASD9; bHLHe6
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ARNTL2; ARNTL2 siRNA (Human); BHLHE6; BMAL2; CLIF; MOP9; PASD9; Aryl hydrocarbon receptor nuclear translocator-like protein 2; Basic-helix-loop-helix-PAS protein MOP9; Brain and muscle ARNT-like 2; CYCLE-like factor; Class E basic helix-loop-helix protein 6; bHLHe6; Member of PAS protein 9; PAS domain-containing protein 9; ARNTL2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ARNTL2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 622

• Applicable Applications for ARNTL2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ARNTL2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ARNTL2 sirna

siRNA to inhibit ARNTL2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 351721418
• NCBI GeneID: 56938
• NCBI Accession #: NP_001234931.1
• NCBI GenBank Nucleotide #: NM_001248002.1
• UniProt Accession #: Q8WYA1; Q8WYA2; Q8WYA3; Q8WYA4; Q96J63; Q9H2M4; Q9NS70; Q9NYQ4; Q9NYQ5; B7Z429; F5H402
• Molecular Weight: 60,390 Da
• NCBI Official Full Name: aryl hydrocarbon receptor nuclear translocator-like protein 2 isoform 2
• NCBI Official Synonym Full Names: aryl hydrocarbon receptor nuclear translocator-like 2
• NCBI Official Symbol: ARNTL2
• NCBI Official Synonym Symbols: CLIF; MOP9; BMAL2; PASD9; bHLHe6
• NCBI Protein Information: aryl hydrocarbon receptor nuclear translocator-like protein 2
• UniProt Protein Name: Aryl hydrocarbon receptor nuclear translocator-like protein 2
• Protein Family: Aryl hydrocarbon receptor nuclear translocator-like protein
• UniProt Gene Name: ARNTL2
• UniProt Synonym Gene Names: BHLHE6; BMAL2; CLIF; MOP9; PASD9; CLIF; bHLHe6
• UniProt Entry Name: BMAL2_HUMAN

NCBI Description

This gene encodes a basic helix-loop-helix transcription factor belonging to the PAS (PER, ARNT, SIM) superfamily. The PAS proteins play important roles in adaptation to low atmospheric and cellular oxygen levels, exposure to certain environmental pollutants, and diurnal oscillations in light and temperature. This protein forms a transcriptionally active heterodimer with the circadian CLOCK protein, the structurally related MOP4, and hypoxia-inducible factors, such as HIF1alpha. Consistent with its role as a biologically relevant partner of circadian and hypoxia factors, this protein is coexpressed in regions of the brain such as the thalamus, hypothalamus, and amygdala. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

ARNTL2: ARNTL2-CLOCK heterodimers activate E-box element (5'- CACGTG-5') transcription. Also, in umbilical vein endothelial cells, activates SERPINE1 through E-box sites. This transactivation is inhibited by PER2 and CRY1. 8 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 12p12.2-p11.2

Cellular Component: transcription factor complex; cytoplasm; nucleolus; nucleus

Molecular Function: protein dimerization activity; signal transducer activity; transcription factor activity

Biological Process: regulation of transcription from RNA polymerase II promoter; circadian rhythm; transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; positive regulation of circadian rhythm; entrainment of circadian clock; signal transduction