ETS2 sirna

ETS2 siRNA (Human)

Cat. Num. AAA8212131

• Gene Names: ETS2; ETS2IT1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ETS2; ETS2 siRNA (Human); Protein C-ets-2; ETS2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ETS2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 469

• Applicable Applications for ETS2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ETS2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ETS2 sirna

siRNA to inhibit ETS2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4885221
• NCBI GeneID: 2114
• NCBI Accession #: NP_005230.1
• NCBI GenBank Nucleotide #: NM_005239.5
• UniProt Accession #: P15036; Q53Y89; A6NM68; D3DSH6
• Molecular Weight: 53,001 Da
• NCBI Official Full Name: protein C-ets-2 isoform 1
• NCBI Official Synonym Full Names: v-ets avian erythroblastosis virus E26 oncogene homolog 2
• NCBI Official Symbol: ETS2
• NCBI Official Synonym Symbols: ETS2IT1
• NCBI Protein Information: protein C-ets-2
• UniProt Protein Name: Protein C-ets-2
• Protein Family: Protein
• UniProt Gene Name: ETS2
• UniProt Entry Name: ETS2_HUMAN

NCBI Description

This gene encodes a transcription factor which regulates genes involved in development and apoptosis. The encoded protein is also a protooncogene and shown to be involved in regulation of telomerase. A pseudogene of this gene is located on the X chromosome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]

Uniprot Description

Ets-2: a proto-oncogenic transcription factor homologous to the v-ets erythroblastosis virus E26 oncogene. A target of the Akt/JNK signaling pathway in macrophages. A PKC pathway activates ETS2, up-regulating GM-CSF in non-small lung carcinoma cells.

Protein type: DNA-binding; Oncoprotein; Transcription factor

Chromosomal Location of Human Ortholog: 21q22.2

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; DNA binding; transcription factor activity; glucocorticoid receptor binding

Biological Process: transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; cell differentiation; skeletal development