MFRP sirna

MFRP siRNA (Human)

Cat. Num. AAA8211919

• Gene Names: MFRP; RD6; NNO2; MCOP5
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MFRP; MFRP siRNA (Human); Membrane frizzled-related protein; Membrane-type frizzled-related protein; MFRP sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: MFRP siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 579

• Applicable Applications for MFRP sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human MFRP gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MFRP sirna

siRNA to inhibit MFRP expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 13899255
• NCBI GeneID: 83552
• NCBI Accession #: NP_113621.1
• NCBI GenBank Nucleotide #: NM_031433.3
• UniProt Accession #: Q9BY79; Q335M3; Q96DQ9; B0YJ36; B0YJ37; B4DHN8
• Molecular Weight: 48,919 Da
• NCBI Official Full Name: membrane frizzled-related protein
• NCBI Official Synonym Full Names: membrane frizzled-related protein
• NCBI Official Symbol: MFRP
• NCBI Official Synonym Symbols: RD6; NNO2; MCOP5
• NCBI Protein Information: membrane frizzled-related protein
• UniProt Protein Name: Membrane frizzled-related protein
• Protein Family: Membrane frizzled-related protein
• UniProt Gene Name: MFRP
• UniProt Entry Name: MFRP_HUMAN

NCBI Description

This gene encodes a member of the frizzled-related protein family. The encoded protein plays an important role in eye development and mutations in this gene have been associated with nanophthalmos, posterior microphthalmia, retinitis pigmentosa, foveoschisis, and optic disc drusen. The protein is encoded by a bicistronic transcript which also encodes C1q and tumor necrosis factor related protein 5 (C1QTNF5). [provided by RefSeq, Jun 2013]

Uniprot Description

MFRP: May play a role in eye development. Defects in MFRP are the cause of nanophthalmos 2 (NNO2). NNO2 is a rare autosomal recessive disorder of eye development characterized by extreme hyperopia and small functional eyes. Defects in MFRP are the cause of microphthalmia isolated type 5 (MCOP5). Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues. Ocular abnormalities like opacities of the cornea and lens, scaring of the retina and choroid, cataract and other abnormalities like cataract may also be present. MCOP5 is characterized by posterior microphthalmia, retinitis pigmentosa, foveoschisis and optic disc drusen.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q23

Cellular Component: apical plasma membrane; integral to membrane

Biological Process: embryonic development

Disease: Microphthalmia, Isolated 5; Nanophthalmos 2