Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit | MYH1 elisa kit

Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit

Cat. Num. AAA2702404

• Gene Names: MYH1; MYHa; HEL71; MYHSA1; MyHC-2x; MyHC-2X/D
• Reactivity: Human
• Synonyms: Myosin Heavy Chain 1; Skeletal Muscle; Adult (MYH1); Human Myosin Heavy Chain 1; Adult (MYH1) ELISA Kit; MYHSA1; MYHa; MyHC-2X/D; Myosin-1; Myosin Heavy Chain IIx/d; MYH1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1).; No significant cross-reactivity or interference between Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) and analogues was observed.
• Sequence Length: 1939

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.059ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MYH1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 115527082
• NCBI GeneID: 4619
• NCBI Accession #: NP_005954.3
• NCBI GenBank Nucleotide #: NM_005963.3
• UniProt Accession #: P12882; Q14CA4; Q9Y622
• Molecular Weight: 223,145 Da
• NCBI Official Full Name: myosin-1
• NCBI Official Synonym Full Names: myosin heavy chain 1
• NCBI Official Symbol: MYH1
• NCBI Official Synonym Symbols: MYHa; HEL71; MYHSA1; MyHC-2x; MyHC-2X/D
• NCBI Protein Information: myosin-1
• UniProt Protein Name: Myosin-1
• UniProt Gene Name: MYH1
• UniProt Synonym Gene Names: MyHC-2x; MyHC-IIx/d

NCBI Description

Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. Myosin heavy chains are encoded by a multigene family. In mammals at least 10 different myosin heavy chain (MYH) isoforms have been described from striated, smooth, and nonmuscle cells. These isoforms show expression that is spatially and temporally regulated during development. [provided by RefSeq, Jul 2008]

Uniprot Description

MYH1: Muscle contraction.

Protein type: Motility/polarity/chemotaxis; Motor

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: cytoplasmic ribonucleoprotein granule; muscle myosin complex; myofibril

Molecular Function: actin filament binding; ATP binding; calmodulin binding; motor activity; protein binding