RAPSN sirna

RAPSN siRNA (Human)

Cat. Num. AAA8211828

• Gene Names: RAPSN; FADS; CMS11; CMS4C; RAPSYN; RNF205
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RAPSN; RAPSN siRNA (Human); RNF205; 43 kDa receptor-associated protein of the synapse; RAPsyn; 43 kDa postsynaptic protein; Acetylcholine receptor-associated 43 kDa protein; RING finger protein 205; RAPSN sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: RAPSN siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 412

• Applicable Applications for RAPSN sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human RAPSN gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RAPSN sirna

siRNA to inhibit RAPSN expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 15619013
• NCBI GeneID: 5913
• NCBI Accession #: NP_005046.2
• NCBI GenBank Nucleotide #: NM_005055.4
• UniProt Accession #: Q13702; Q8TDF3; Q9BTD9
• Molecular Weight: 39,912 Da
• NCBI Official Full Name: 43 kDa receptor-associated protein of the synapse isoform 1
• NCBI Official Synonym Full Names: receptor-associated protein of the synapse
• NCBI Official Symbol: RAPSN
• NCBI Official Synonym Symbols: FADS; CMS11; CMS4C; RAPSYN; RNF205
• NCBI Protein Information: 43 kDa receptor-associated protein of the synapse
• UniProt Protein Name: 43 kDa receptor-associated protein of the synapse
• Protein Family: 43 kDa receptor-associated protein of the synapse
• UniProt Gene Name: RAPSN
• UniProt Synonym Gene Names: RNF205; RAPsyn
• UniProt Entry Name: RAPSN_HUMAN

NCBI Description

This gene encodes a member of a family of proteins that are receptor associated proteins of the synapse. The encoded protein contains a conserved cAMP-dependent protein kinase phosphorylation site, and plays a critical role in clustering and anchoring nicotinic acetylcholine receptors at synaptic sites by linking the receptors to the underlying postsynaptic cytoskeleton, possibly by direct association with actin or spectrin. Mutations in this gene may play a role in postsynaptic congenital myasthenic syndromes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Apr 2011]

Uniprot Description

RAPSN: Thought to play some role in anchoring or stabilizing the nicotinic acetylcholine receptor at synaptic sites. It may link the receptor to the underlying postsynaptic cytoskeleton, possibly by direct association with actin or spectrin. Defects in RAPSN are a cause of congenital myasthenic syndrome with acetylcholine receptor deficiency (CMS-ACHRD). A postsynaptic congenital myasthenic syndrome. Congenital myasthenic syndromes (CMS) are inherited disorders of neuromuscular transmission that stem from mutations in presynaptic, synaptic, or postsynaptic proteins. Defects in RAPSN are the cause of fetal akinesia deformation sequence (FADS); also known as Pena- Shokeir syndrome type 1 or fetal akinesia sequence or arthrogryposis multiplex congenita with pulmonary hypoplasia. FADS is a rare condition characterized by decreased intrauterine fetal movement, congenital limb contractures, pulmonary hypoplasia, polyhydramnios and craniofacial abnormalities. Belongs to the RAPsyn family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; Motility/polarity/chemotaxis; Cell adhesion

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: Golgi apparatus; centrosome; postsynaptic membrane; cytoplasm; plasma membrane; cell junction; neuromuscular junction

Molecular Function: ionotropic glutamate receptor binding; zinc ion binding; protein anchor; acetylcholine receptor binding

Biological Process: synaptic transmission; positive regulation of neuron apoptosis; synaptic transmission, cholinergic

Disease: Myasthenic Syndrome, Congenital, 11, Associated With Acetylcholine Receptor Deficiency; Fetal Akinesia Deformation Sequence