PDE1B sirna

PDE1B siRNA (Human)

Cat. Num. AAA8211478

• Gene Names: PDE1B; PDE1B1; PDES1B; HEL-S-79p
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PDE1B; PDE1B siRNA (Human); PDE1B1; PDES1B; Calcium/calmodulin-dependent 3'.5'-cyclic nucleotide phosphodiesterase 1B; Cam-PDE 1B; 63 kDa Cam-PDE; PDE1B sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PDE1B siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 536

• Applicable Applications for PDE1B sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PDE1B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PDE1B sirna

siRNA to inhibit PDE1B expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4505677
• NCBI GeneID: 5153
• NCBI Accession #: NP_000915.1
• NCBI GenBank Nucleotide #: NM_000924.3
• UniProt Accession #: Q01064; Q92825; Q96KP3
• Molecular Weight: 59,051 Da
• NCBI Official Full Name: calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B isoform 1
• NCBI Official Synonym Full Names: phosphodiesterase 1B, calmodulin-dependent
• NCBI Official Symbol: PDE1B
• NCBI Official Synonym Symbols: PDE1B1; PDES1B; HEL-S-79p
• NCBI Protein Information: calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B
• UniProt Protein Name: Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B
• UniProt Gene Name: PDE1B
• UniProt Synonym Gene Names: PDE1B1; PDES1B; Cam-PDE 1B
• UniProt Entry Name: PDE1B_HUMAN

NCBI Description

The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE1 subfamily. Members of the PDE1 family are calmodulin-dependent PDEs that are stimulated by a calcium-calmodulin complex. This PDE has dual-specificity for the second messengers, cAMP and cGMP, with a preference for cGMP as a substrate. cAMP and cGMP function as key regulators of many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011]

Uniprot Description

PDE1B: Cyclic nucleotide phosphodiesterase with a dual- specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a preference for cGMP as a substrate. Belongs to the cyclic nucleotide phosphodiesterase family. PDE1 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleotide Metabolism - purine; EC 3.1.4.17; Phosphodiesterase; Apoptosis

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: cell soma; cytosol

Molecular Function: calmodulin binding; 3',5'-cyclic-AMP phosphodiesterase activity; calmodulin-dependent cyclic-nucleotide phosphodiesterase activity; calcium- and calmodulin-regulated 3',5'-cyclic-GMP phosphodiesterase activity; metal ion binding

Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; monocyte differentiation; nerve growth factor receptor signaling pathway; apoptosis; regulation of neurotransmitter levels; cAMP catabolic process; response to amphetamine; regulation of dopamine metabolic process; locomotory behavior; signal transduction; phospholipase C activation; visual learning; innate immune response; cGMP catabolic process; blood coagulation; serotonin metabolic process