WDR82 sirna

WDR82 siRNA (Human)

Cat. Num. AAA8211473

• Gene Names: WDR82; SWD2; MST107; WDR82A; MSTP107; PRO2730; TMEM113; PRO34047
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: WDR82; WDR82 siRNA (Human); TMEM113; WDR82A; WD repeat-containing protein 82; Protein TMEM113; Swd2; WDR82 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: WDR82 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 313

• Applicable Applications for WDR82 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human WDR82 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for WDR82 sirna

siRNA to inhibit WDR82 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 147904340
• NCBI GeneID: 80335
• NCBI Accession #: NP_079498.2
• NCBI GenBank Nucleotide #: NM_025222.3
• UniProt Accession #: Q6UXN9; Q8TEB2; A8K5R5
• Molecular Weight: 35,079 Da
• NCBI Official Full Name: WD repeat-containing protein 82
• NCBI Official Synonym Full Names: WD repeat domain 82
• NCBI Official Symbol: WDR82
• NCBI Official Synonym Symbols: SWD2; MST107; WDR82A; MSTP107; PRO2730; TMEM113; PRO34047
• NCBI Protein Information: WD repeat-containing protein 82
• UniProt Protein Name: WD repeat-containing protein 82
• Protein Family: WD repeat-containing protein
• UniProt Gene Name: WDR82
• UniProt Synonym Gene Names: TMEM113; WDR82A
• UniProt Entry Name: WDR82_HUMAN

NCBI Description

TMEM113 (WDR82) is a component of the mammalian SET1A (MIM 611052)/SET1B (MIM 611055) histone H3-Lys4 methyltransferase complexes (Lee and Skalnik, 2005 [PubMed 16253997]; Lee et al., 2007 [PubMed 17355966]).[supplied by OMIM, Jul 2010]

Uniprot Description

WDR82: a WD repeat-containing protein. A regulatory component of the histone H3K4 methylatransferase SET1 complex implicated in tethering this complex to transcriptional start sites of active genes. Facilitates histone H3K4 methylation via recruitment of the SETD1A or SETD1B to phospho-S5 in the C-terminal domain (CTD) of RNA polymerase II large subunit (POLR2A). Component of PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. Component of the SET1 complex, composed of at least the catalytic subunit (SETD1A or SETD1B), WDR5, WDR82, RBBP5, ASH2L/ASH2, CXXC1/CFP1, HCFC1 and DPY30. Component of the PTW/PP1 phosphatase complex, composed of PPP1R10/PNUTS, TOX4, WDR82, and PPP1CA, ¿B, or -C. Associated with multiple protein complexes including an RNA polymerase II complex, MLL3/MLL4 complex and a chaperonin-containing TCP1 complex. Interacts with CUL4B. Interacts with RBBP5 and SETD1B. Interacts with SETD1A (via RRM domain). Interacts with POLR2B. Interacts with hyperphosphorylated C-terminal domain (CTD) of RNA polymerase II large subunit (POLR2A). SETD1A enhances its interaction with POLR2A. Interacts with PPP1R10/PNUTS. Interacts with PPP1CA in the presence of PPP1R10/PNUTS.

Protein type: Methyltransferase

Chromosomal Location of Human Ortholog: 3p21.2

Cellular Component: histone methyltransferase complex; chromatin

Molecular Function: protein binding; histone lysine N-methyltransferase activity (H3-K4 specific); chromatin binding

Biological Process: histone H3-K4 methylation