CRHR1 sirna

CRHR1 siRNA (Mouse)

Cat. Num. AAA8211161

• Gene Names: Crhr1; Crhr; CRFR1
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CRHR1; CRHR1 siRNA (Mouse); CRHR; Corticotropin-releasing factor receptor 1; CRF-R-1; CRF-R1; CRFR-1; Corticotropin-releasing hormone receptor 1; CRH-R-1; CRH-R1; CRHR1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: CRHR1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 415

• Applicable Applications for CRHR1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CRHR1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CRHR1 sirna

siRNA to inhibit CRHR1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6681013
• NCBI GeneID: 12921
• NCBI Accession #: NP_031788.1
• NCBI GenBank Nucleotide #: NM_007762.4
• UniProt Accession #: P35347
• Molecular Weight: 47,769 Da
• NCBI Official Full Name: corticotropin-releasing factor receptor 1
• NCBI Official Synonym Full Names: corticotropin releasing hormone receptor 1
• NCBI Official Symbol: Crhr1
• NCBI Official Synonym Symbols: Crhr; CRFR1
• NCBI Protein Information: corticotropin-releasing factor receptor 1
• UniProt Protein Name: Corticotropin-releasing factor receptor 1
• Protein Family: Corticotropin-releasing factor receptor
• UniProt Gene Name: Crhr1
• UniProt Synonym Gene Names: Crhr; CRF-R-1; CRF-R1; CRFR-1; CRH-R-1; CRH-R1
• UniProt Entry Name: CRFR1_MOUSE

NCBI Description

The protein encoded by this gene is a G-protein coupled receptor that binds corticotropin-releasing factor (CRH) and urocortin (UCN) with high affinity. The encoded protein upregulates adenylate cyclase activity and intracellular cAMP levels. This protein is essential for the activation of signal transduction pathways that regulate diverse physiological processes including stress, reproduction, immune response, and obesity. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]

Uniprot Description

CRF-R1: a G-protein coupled receptor that specifically binds corticotropin-releasing hormone, a potent mediator of endocrine, autonomic, behavioral, and immune responses to stress. Four splice-variant isoforms have been described.

Protein type: GPCR, family 2; Membrane protein, multi-pass; Membrane protein, integral; Receptor, GPCR

Cellular Component: cell; dendrite; integral to membrane; trans-Golgi network; cytosol; intrinsic to plasma membrane; multivesicular body; membrane; cell soma; apical part of cell; plasma membrane; vesicle; endosome

Molecular Function: G-protein coupled receptor activity; signal transducer activity; transmembrane receptor activity; corticotropin-releasing hormone binding; corticotropin-releasing hormone receptor activity; protein complex binding; G-protein alpha-subunit binding; corticotrophin-releasing factor receptor activity; peptide binding

Biological Process: fear response; hormone-mediated signaling; regulation of synaptic plasticity; behavioral response to ethanol; signal transduction; G-protein signaling, adenylate cyclase activating pathway; elevation of cytosolic calcium ion concentration; behavioral response to pain; general adaptation syndrome, behavioral process; cell surface receptor linked signal transduction; epithelial cell differentiation; behavioral response to cocaine; neuropeptide signaling pathway; positive regulation of cAMP biosynthetic process; visual learning; feeding behavior; response to electrical stimulus; adrenal gland development; positive regulation of mast cell degranulation; memory; G-protein signaling, coupled to cAMP nucleotide second messenger; G-protein coupled receptor protein signaling pathway; adrenocorticotropin hormone secretion; response to hypoxia; negative regulation of epinephrine secretion; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating)