ASIC2 sirna

ASIC2 siRNA (Mouse)

Cat. Num. AAA8210849

• Gene Names: Asic2; BNC1; Mdeg; ACIC2; Accn1; BNaC1; BNaC1a
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ASIC2; ASIC2 siRNA (Mouse); ACCN1; BNAC1; Acid-sensing ion channel 2; Amiloride-sensitive brain sodium channel; Amiloride-sensitive cation channel 1. neuronal; Brain sodium channel 1; BNC1; BNaC1; ASIC2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: ASIC2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 563

• Applicable Applications for ASIC2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ASIC2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ASIC2 sirna

siRNA to inhibit ASIC2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 77404288
• NCBI GeneID: 11418
• NCBI Accession #: NP_001029185.1
• NCBI GenBank Nucleotide #: NM_001034013.2
• UniProt Accession #: Q925H0; Q5SUU2; Q61203
• Molecular Weight: 63,204 Da
• NCBI Official Full Name: acid-sensing ion channel 2 isoform MDEG1
• NCBI Official Synonym Full Names: acid-sensing (proton-gated) ion channel 2
• NCBI Official Symbol: Asic2
• NCBI Official Synonym Symbols: BNC1; Mdeg; ACIC2; Accn1; BNaC1; BNaC1a
• NCBI Protein Information: acid-sensing ion channel 2
• UniProt Protein Name: Acid-sensing ion channel 2
• Protein Family: Acid-sensing ion channel
• UniProt Gene Name: Asic2
• UniProt Synonym Gene Names: Accn1; Bnac1; ASIC2; BNC1; BNaC1
• UniProt Entry Name: ASIC2_MOUSE

Uniprot Description

ASIC2: Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Also permeable for Li(+) and K(+). Generates a biphasic current with a fast inactivating and a slow sustained phase. Heteromeric channel assembly seems to modulate. Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. ASIC2 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter, ion channel; Channel, cation; Membrane protein, multi-pass; Transporter; Apoptosis; Membrane protein, integral

Cellular Component: neuron projection; cell soma; membrane; integral to plasma membrane; dendritic spine; integral to membrane; plasma membrane; synapse

Molecular Function: amiloride-sensitive sodium channel activity; protein binding; ion gated channel activity; voltage-gated sodium channel activity; ion channel activity; cation channel activity; sodium channel activity

Biological Process: regulation of systemic arterial blood pressure by aortic arch baroreceptor feedback; sodium ion transport; sensory perception of sour taste; detection of mechanical stimulus involved in sensory perception; positive regulation of synaptogenesis; regulation of membrane potential; sensory perception of sound; transport; response to mechanical stimulus; regulation of gene expression; regulation of vasoconstriction; regulation of blood coagulation; phototransduction; ion transport; response to acidity; cation transport; negative regulation of apoptosis