ARL6IP5 sirna

ARL6IP5 siRNA (Human)

Cat. Num. AAA8210119

• Gene Names: ARL6IP5; JWA; jmx; hp22; PRAF3; DERP11; HSPC127; addicsin; GTRAP3-18
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ARL6IP5; ARL6IP5 siRNA (Human); DERP11; JWA; PRA2; PRAF3; PRA1 family protein 3; ADP-ribosylation factor-like protein 6-interacting protein 5; ARL-6-interacting protein 5; Aip-5; Cytoskeleton-related vitamin A-responsive protein; Dermal papilla-derived protein 11; GTRAP3-18; Glutamate transporter EAAC1-interacting protein; JM5; Prenylated Rab acceptor protein 2; Protein JWa; Putative MAPK-activating protein PM27; ARL6IP5 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ARL6IP5 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 188

• Applicable Applications for ARL6IP5 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ARL6IP5 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ARL6IP5 sirna

siRNA to inhibit ARL6IP5 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 5453704
• NCBI GeneID: 10550
• NCBI Accession #: NP_006398.1
• NCBI GenBank Nucleotide #: NM_006407.3
• UniProt Accession #: O75915; Q53ES3; Q5KU08; B2R6V5
• Molecular Weight: 21,615 Da
• NCBI Official Full Name: PRA1 family protein 3
• NCBI Official Synonym Full Names: ADP-ribosylation factor-like 6 interacting protein 5
• NCBI Official Symbol: ARL6IP5
• NCBI Official Synonym Symbols: JWA; jmx; hp22; PRAF3; DERP11; HSPC127; addicsin; GTRAP3-18
• NCBI Protein Information: PRA1 family protein 3
• UniProt Protein Name: PRA1 family protein 3
• Protein Family: PRA1 family protein
• UniProt Gene Name: ARL6IP5
• UniProt Synonym Gene Names: DERP11; JWA; PRA2; PRAF3; ARL-6-interacting protein 5; Aip-5
• UniProt Entry Name: PRAF3_HUMAN

NCBI Description

Expression of this gene is affected by vitamin A. The encoded protein of this gene may be associated with the cytoskeleton. A similar protein in rats may play a role in the regulation of cell differentiation. The rat protein binds and inhibits the cell membrane glutamate transporter EAAC1. The expression of the rat gene is upregulated by retinoic acid, which results in a specific reduction in EAAC1-mediated glutamate transport. [provided by RefSeq, Jul 2008]

Uniprot Description

ARL6IP5: Regulates intracellular concentrations of taurine and glutamate. Negatively modulates SLC1A1/EAAC1 glutamate transport activity by decreasing its affinity for glutamate. May be involved in membrane traffic. Belongs to the PRA1 family.

Protein type: Endoplasmic reticulum; Membrane protein, integral; Cytoskeletal; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 3p14

Cellular Component: endoplasmic reticulum membrane; membrane; endoplasmic reticulum; integral to membrane

Molecular Function: protein C-terminus binding

Biological Process: L-glutamate transport; positive regulation of apoptosis; positive regulation of caspase activity; negative regulation of transport; induction of apoptosis by oxidative stress; positive regulation of stress-activated MAPK cascade