Typical Testing Data/Standard Curve (for reference only)
Human PRA1 family protein 3 (ARL6IP5) ELISA Kit | ARL6IP5 elisa kit
Human PRA1 family protein 3 (ARL6IP5) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: ARL6IP5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ARL6IP5 antibody and an ARL6IP5-HRP conjugate. The assay sample and buffer are incubated together with ARL6IP5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ARL6IP5 concentration since ARL6IP5 from samples and ARL6IP5-HRP conjugate compete for the anti-ARL6IP5 antibody binding site. Since the number of sites is limited, as more sites are occupied by ARL6IP5 from the sample, fewer sites are left to bind ARL6IP5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ARL6IP5 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
Expression of this gene is affected by vitamin A. The encoded protein of this gene may be associated with the cytoskeleton. A similar protein in rats may play a role in the regulation of cell differentiation. The rat protein binds and inhibits the cell membrane glutamate transporter EAAC1. The expression of the rat gene is upregulated by retinoic acid, which results in a specific reduction in EAAC1-mediated glutamate transport. [provided by RefSeq, Jul 2008]
Uniprot Description
ARL6IP5: Regulates intracellular concentrations of taurine and glutamate. Negatively modulates SLC1A1/EAAC1 glutamate transport activity by decreasing its affinity for glutamate. May be involved in membrane traffic. Belongs to the PRA1 family.
Protein type: Membrane protein, multi-pass; Cytoskeletal; Membrane protein, integral; Endoplasmic reticulum
Chromosomal Location of Human Ortholog: 3p14
Cellular Component: endoplasmic reticulum membrane; membrane; endoplasmic reticulum; integral to membrane
Molecular Function: protein C-terminus binding
Biological Process: L-glutamate transport; positive regulation of apoptosis; positive regulation of caspase activity; negative regulation of transport; induction of apoptosis by oxidative stress; positive regulation of stress-activated MAPK cascade
Research Articles on ARL6IP5
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Product Notes
The Human ARL6IP5 arl6ip5 (Catalog #AAA7207293) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7207293 ELISA Kit recognizes Human ARL6IP5. It is sometimes possible for the material contained within the vial of "PRA1 family protein 3 (ARL6IP5), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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