APOA2 sirna

APOA2 siRNA (Mouse)

Cat. Num. AAA8210085

• Gene Names: Apoa2; Alp-2; Hdl-1; ApoAII; Apoa-2; Apo-AII; ApoA-II
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: APOA2; APOA2 siRNA (Mouse); Apolipoprotein A-II; Apo-AII; ApoA-II; Apolipoprotein A2; APOA2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: APOA2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 102

• Applicable Applications for APOA2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse APOA2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for APOA2 sirna

siRNA to inhibit APOA2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 771916686
• NCBI GeneID: 11807
• NCBI Accession #: NP_001292478.1
• NCBI GenBank Nucleotide #: NM_001305549.1
• UniProt Accession #: P09813; Q3UKX6; Q61317
• Molecular Weight: 11,309 Da
• NCBI Official Full Name: apolipoprotein A-II preproprotein
• NCBI Official Synonym Full Names: apolipoprotein A-II
• NCBI Official Symbol: Apoa2
• NCBI Official Synonym Symbols: Alp-2; Hdl-1; ApoAII; Apoa-2; Apo-AII; ApoA-II
• NCBI Protein Information: apolipoprotein A-II
• UniProt Protein Name: Apolipoprotein A-II
• Protein Family: Apolipoprotein
• UniProt Gene Name: Apoa2
• UniProt Synonym Gene Names: Apo-AII; ApoA-II; ProapoA-II
• UniProt Entry Name: APOA2_MOUSE

NCBI Description

This gene encodes a component of high density lipoproteins (HDL). Mice lacking the encoded protein have low HDL-cholesterol levels, smaller HDL particles, increased clearance of triglyceride-rich lipoproteins and insulin hypersensitivity. Transgenic mice overexpressing the encoded protein have elevated levels of HDL-cholesterol and show increased susceptibility to atherosclerosis. Alternative splicing of this gene results in multiple variants. [provided by RefSeq, Mar 2015]

Uniprot Description

APOA2: May stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism. Belongs to the apolipoprotein A2 family.

Protein type: Lipid-binding; Secreted, signal peptide; Secreted; Endoplasmic reticulum

Cellular Component: extracellular space; chylomicron; extracellular region

Molecular Function: lipid transporter activity; protein homodimerization activity; lipase inhibitor activity; protein heterodimerization activity; cholesterol transporter activity; cholesterol binding; phospholipid binding; phosphatidylcholine binding; lipid binding; high-density lipoprotein binding; apolipoprotein receptor binding

Biological Process: positive regulation of catalytic activity; protein folding; negative regulation of cholesterol transport; regulation of protein stability; diacylglycerol catabolic process; positive regulation of lipid catabolic process; fatty acid metabolic process; negative regulation of lipase activity; phospholipid efflux; regulation of cholesterol absorption; negative regulation of lipid catabolic process; transport; response to glucose stimulus; cholesterol metabolic process; organ regeneration; cholesterol transport; protein amino acid oxidation; phospholipid catabolic process; beta-glucoside transport; positive regulation of interleukin-8 biosynthetic process; lipoprotein metabolic process; cholesterol efflux; lipid transport; negative regulation of cytokine secretion during immune response; cholesterol homeostasis; reverse cholesterol transport; peptidyl-methionine modification; phosphatidylcholine biosynthetic process