LIN7C sirna

LIN7C siRNA (Human)

Cat. Num. AAA8209877

• Gene Names: LIN7C; MALS3; VELI3; LIN-7C; MALS-3; LIN-7-C
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: LIN7C; LIN7C siRNA (Human); MALS3; VELI3; Protein lin-7 homolog C; Lin-7C; Mammalian lin-seven protein 3; MALS-3; Vertebrate lin-7 homolog 3; Veli-3; LIN7C sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: LIN7C siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 197

• Applicable Applications for LIN7C sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human LIN7C gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for LIN7C sirna

siRNA to inhibit LIN7C expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 8922944
• NCBI GeneID: 55327
• NCBI Accession #: NP_060832.1
• NCBI GenBank Nucleotide #: NM_018362.3
• UniProt Accession #: Q9NUP9
• Molecular Weight: 21,834 Da
• NCBI Official Full Name: protein lin-7 homolog C
• NCBI Official Synonym Full Names: lin-7 homolog C (C. elegans)
• NCBI Official Symbol: LIN7C
• NCBI Official Synonym Symbols: MALS3; VELI3; LIN-7C; MALS-3; LIN-7-C
• NCBI Protein Information: protein lin-7 homolog C
• UniProt Protein Name: Protein lin-7 homolog C
• Protein Family: Protein
• UniProt Gene Name: LIN7C
• UniProt Synonym Gene Names: MALS3; VELI3; Lin-7C; MALS-3; Veli-3
• UniProt Entry Name: LIN7C_HUMAN

Uniprot Description

LIN7C: Plays a role in establishing and maintaining the asymmetric distribution of channels and receptors at the plasma membrane of polarized cells. Forms membrane-associated multiprotein complexes that may regulate delivery and recycling of proteins to the correct membrane domains. The tripartite complex composed of LIN7 (LIN7A, LIN7B or LIN7C), CASK and APBA1 may have the potential to couple synaptic vesicle exocytosis to cell adhesion in brain. Ensures the proper localization of GRIN2B (subunit 2B of the NMDA receptor) to neuronal postsynaptic density and may function in localizing synaptic vesicles at synapses where it is recruited by beta-catenin and cadherin. Required to localize Kir2 channels, GABA transporter (SLC6A12) and EGFR/ERBB1, ERBB2, ERBB3 and ERBB4 to the basolateral membrane of epithelial cells. Belongs to the lin-7 family.

Protein type: Cell adhesion

Chromosomal Location of Human Ortholog: 11p14

Cellular Component: postsynaptic membrane; neuron projection; tight junction; basolateral plasma membrane; cytoplasm; postsynaptic density; plasma membrane; synapse; intercellular junction

Molecular Function: protein domain specific binding; cytoskeletal protein binding; PDZ domain binding

Biological Process: asymmetric protein localization; protein transport; exocytosis; neurotransmitter secretion; morphogenesis of an epithelial sheet; maintenance of epithelial cell polarity