BCL9 sirna

BCL9 siRNA (Mouse)

Cat. Num. AAA8209519

• Gene Names: Bcl9; Gm130; 2610202E01Rik; 8030475K17Rik; A330041G23Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: BCL9; BCL9 siRNA (Mouse); B-cell CLL/lymphoma 9 protein; B-cell lymphoma 9 protein; Bcl-9; BCL9 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: BCL9 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1425

• Applicable Applications for BCL9 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse BCL9 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for BCL9 sirna

siRNA to inhibit BCL9 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 56606137
• NCBI GeneID: 77578
• NCBI Accession #: NP_084209.3
• NCBI GenBank Nucleotide #: NM_029933.4
• UniProt Accession #: Q9D219; Q67FX9; Q8BUJ8; Q8VE74; B2RQC0
• Molecular Weight: 148,971 Da
• NCBI Official Full Name: B-cell CLL/lymphoma 9 protein
• NCBI Official Synonym Full Names: B cell CLL/lymphoma 9
• NCBI Official Symbol: Bcl9
• NCBI Official Synonym Symbols: Gm130; 2610202E01Rik; 8030475K17Rik; A330041G23Rik
• NCBI Protein Information: B-cell CLL/lymphoma 9 protein
• UniProt Protein Name: B-cell CLL/lymphoma 9 protein
• Protein Family: B-cell CLL/lymphoma 9 protein
• UniProt Gene Name: Bcl9
• UniProt Synonym Gene Names: B-cell lymphoma 9 protein; Bcl-9
• UniProt Entry Name: BCL9_MOUSE

Uniprot Description

Bcl-9: Involved in signal transduction through the Wnt pathway. Promotes beta-catenin's transcriptional activity. A chromosomal aberration involving BCL9 is found in a patient with precusor B-cell acute lymphoblastic leukemia (ALL). Translocation t(1;14)(q21;q32). This translocation leaves the coding region intact, but may have pathogenic effects due to alterations in the expression level of BCL9. Several cases of translocations within the 3'-UTR of BCL9 have been found in B-cell malignancies. Belongs to the BCL9 family.

Protein type: Transcription factor; Oncoprotein

Cellular Component: Golgi apparatus; cis-Golgi network; cytoplasm; nucleus

Molecular Function: beta-catenin binding

Biological Process: Wnt receptor signaling pathway; somatic stem cell maintenance; myotube differentiation involved in skeletal muscle regeneration; positive regulation of transcription from RNA polymerase II promoter; Wnt receptor signaling pathway through beta-catenin