DDX11 sirna

DDX11 siRNA (Human)

Cat. Num. AAA8209012

• Gene Names: DDX11; CHL1; KRG2; WABS; CHLR1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DDX11; DDX11 siRNA (Human); CHL1; CHLR1; KRG2; Probable ATP-dependent RNA helicase DDX11; CHL1-related protein 1; hCHLR1; DEAD/H box protein 11; Keratinocyte growth factor-regulated gene 2 protein; KRG-2; DDX11 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: DDX11 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 970

• Applicable Applications for DDX11 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human DDX11 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DDX11 sirna

siRNA to inhibit DDX11 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 380420356
• NCBI GeneID: 1663
• NCBI Accession #: NP_001244073.1
• NCBI GenBank Nucleotide #: NM_001257144.1
• UniProt Accession #: Q96FC9; Q13333; Q86VQ4; Q86W62; Q92498; Q92770; Q92998; Q92999
• Molecular Weight: 32,951 Da
• NCBI Official Full Name: probable ATP-dependent RNA helicase DDX11 isoform 3
• NCBI Official Synonym Full Names: DEAD/H (Asp-Glu-Ala-Asp/His) box helicase 11
• NCBI Official Symbol: DDX11
• NCBI Official Synonym Symbols: CHL1; KRG2; WABS; CHLR1
• NCBI Protein Information: probable ATP-dependent RNA helicase DDX11
• UniProt Protein Name: Probable ATP-dependent RNA helicase DDX11
• Protein Family: ATP-dependent DNA helicase
• UniProt Gene Name: DDX11
• UniProt Synonym Gene Names: CHL1; CHLR1; KRG2; hCHLR1; KRG-2
• UniProt Entry Name: DDX11_HUMAN

NCBI Description

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is an enzyme that possesses both ATPase and DNA helicase activities. This gene is a homolog of the yeast CHL1 gene, and may function to maintain chromosome transmission fidelity and genome stability. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

DDX11: DNA helicase involved in cellular proliferation. Possesses DNA-dependent ATPase and helicase activities. This helicase translocates on single-stranded DNA in the 5' to 3' direction in the presence of ATP and, to a lesser extent, dATP. Its unwinding activity requires a 5'-single-stranded region for helicase loading, since flush-ended duplex structures do not support unwinding. The helicase activity is capable of displacing duplex regions up to 100 bp, which can be extended to 500 bp by RPA or the cohesion establishment factor, the Ctf18-RFC (replication factor C) complex activities. Stimulates the flap endonuclease activity of FEN1. Required for normal sister chromatid cohesion. Required for recruitment of bovine papillomavirus type 1 regulatory protein E2 to mitotic chrmosomes and for viral genome maintenance. Required for maintaining the chromosome segregation and is essential for embryonic development and the prevention of aneuploidy. May function during either S, G2, or M phase of the cell cycle. Binds to both single- and double-stranded DNA. Defects in DDX11 are the cause of Warsaw breakage syndrome (WBRS). It is a syndrome characterized by severe microcephaly, pre- and postnatal growth retardation, facial dysmorphism and abnormal skin pigmentation. Additional features include high arched palate, coloboma of the right optic disk, deafness, ventricular septal defect, toes and fingers abnormalities. At cellular level, drug-induced chromosomal breakage, a feature of Fanconi anemia, and sister chromatid cohesion defects, a feature of Roberts syndrome, coexist. Belongs to the DEAD box helicase family. DEAH subfamily. DDX11/CHL1 sub-subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.6.4.13; Nucleolus; Helicase

Chromosomal Location of Human Ortholog: 12p11

Cellular Component: nucleoplasm; spindle pole; nuclear chromatin; cytoplasm; nucleolus; midbody; nucleus

Molecular Function: ATP-dependent DNA helicase activity; DNA-dependent ATPase activity; protein binding; RNA binding; metal ion binding; 4 iron, 4 sulfur cluster binding; double-stranded DNA binding; helicase activity; single-stranded DNA binding; ATP binding

Biological Process: unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; viral reproduction; unfolded protein response; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; sister chromatid cohesion; DNA duplex unwinding

Disease: Warsaw Breakage Syndrome