Rabbit anti-Human DDX11 Polyclonal Antibody | anti-DDX11 antibody

DDX11 Antibody

Cat. Num. AAA9404364

• Gene Names: DDX11; CHL1; KRG2; WABS; CHLR1
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Antigen affinity purification.
• Synonyms: DDX11; Polyclonal Antibody; DDX11 Antibody; CHL1; KRG2; WABS; CHLR1; anti-DDX11 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total DDX11 protein.
• Purity/Purification: Antigen affinity purification.
• Form/Format: Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.
• Concentration: 1.2 mg/ml (varies by lot)
• Sequence Length: 970

• Applicable Applications for anti-DDX11 antibody: Immunohistochemistry (IHC)
• Application Notes: Immunohistochemistry: 1:50-1:200
• Immunogen Type: Recombinant Protein
• Immunogen Description: Fusion protein corresponding to a region derived from internal residues of human DEAD/H (Asp-Glu-Ala-Asp/His) box helicase 11
• Target Name: DDX11
• Preparation and Storage: Store at -20 degree C

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissue using at dilution 1/30.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human brain cancer tissue using at dilution 1/30.)

Related Product Information for anti-DDX11 antibody

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is an enzyme that possesses both ATPase and DNA helicase activities. This gene is a homolog of the yeast CHL1 gene, and may function to maintain chromosome transmission fidelity and genome stability. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Product Categories/Family for anti-DDX11 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 380420356
• NCBI GeneID: 1663
• NCBI Accession #: NP_001244073.1
• NCBI GenBank Nucleotide #: NM_001257144.1
• UniProt Accession #: Q96FC9; Q13333; Q86VQ4; Q86W62; Q92498; Q92770; Q92998; Q92999
• Molecular Weight: 32,951 Da
• NCBI Official Full Name: probable ATP-dependent RNA helicase DDX11 isoform 3
• NCBI Official Synonym Full Names: DEAD/H (Asp-Glu-Ala-Asp/His) box helicase 11
• NCBI Official Symbol: DDX11
• NCBI Official Synonym Symbols: CHL1; KRG2; WABS; CHLR1
• NCBI Protein Information: probable ATP-dependent RNA helicase DDX11
• UniProt Protein Name: Probable ATP-dependent RNA helicase DDX11
• Protein Family: ATP-dependent DNA helicase
• UniProt Gene Name: DDX11
• UniProt Synonym Gene Names: CHL1; CHLR1; KRG2; hCHLR1; KRG-2
• UniProt Entry Name: DDX11_HUMAN

NCBI Description

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is an enzyme that possesses both ATPase and DNA helicase activities. This gene is a homolog of the yeast CHL1 gene, and may function to maintain chromosome transmission fidelity and genome stability. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

DDX11: DNA helicase involved in cellular proliferation. Possesses DNA-dependent ATPase and helicase activities. This helicase translocates on single-stranded DNA in the 5' to 3' direction in the presence of ATP and, to a lesser extent, dATP. Its unwinding activity requires a 5'-single-stranded region for helicase loading, since flush-ended duplex structures do not support unwinding. The helicase activity is capable of displacing duplex regions up to 100 bp, which can be extended to 500 bp by RPA or the cohesion establishment factor, the Ctf18-RFC (replication factor C) complex activities. Stimulates the flap endonuclease activity of FEN1. Required for normal sister chromatid cohesion. Required for recruitment of bovine papillomavirus type 1 regulatory protein E2 to mitotic chrmosomes and for viral genome maintenance. Required for maintaining the chromosome segregation and is essential for embryonic development and the prevention of aneuploidy. May function during either S, G2, or M phase of the cell cycle. Binds to both single- and double-stranded DNA. Defects in DDX11 are the cause of Warsaw breakage syndrome (WBRS). It is a syndrome characterized by severe microcephaly, pre- and postnatal growth retardation, facial dysmorphism and abnormal skin pigmentation. Additional features include high arched palate, coloboma of the right optic disk, deafness, ventricular septal defect, toes and fingers abnormalities. At cellular level, drug-induced chromosomal breakage, a feature of Fanconi anemia, and sister chromatid cohesion defects, a feature of Roberts syndrome, coexist. Belongs to the DEAD box helicase family. DEAH subfamily. DDX11/CHL1 sub-subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.6.4.13; Helicase; Nucleolus

Chromosomal Location of Human Ortholog: 12p11

Cellular Component: nucleoplasm; spindle pole; nuclear chromatin; cytoplasm; nucleolus; midbody; nucleus

Molecular Function: ATP-dependent DNA helicase activity; DNA-dependent ATPase activity; protein binding; RNA binding; 4 iron, 4 sulfur cluster binding; metal ion binding; double-stranded DNA binding; helicase activity; ATP binding; single-stranded DNA binding

Biological Process: unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; viral reproduction; unfolded protein response; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; sister chromatid cohesion; DNA duplex unwinding

Disease: Warsaw Breakage Syndrome