ITGAM sirna

ITGAM siRNA (Human)

Cat. Num. AAA8208773

• Gene Names: ITGAM; CR3A; MO1A; CD11B; MAC-1; MAC1A; SLEB6
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ITGAM; ITGAM siRNA (Human); CD11B; CR3A; Integrin alpha-M; CD11 antigen-like family member B; CR-3 alpha chain; Cell surface glycoprotein MAC-1 subunit alpha; Leukocyte adhesion receptor MO1; Neutrophil adherence receptor; CD antigen CD11b; ITGAM sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ITGAM siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1152

• Applicable Applications for ITGAM sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ITGAM gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ITGAM sirna

siRNA to inhibit ITGAM expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 88501734
• NCBI GeneID: 3684
• NCBI Accession #: NP_000623.2
• NCBI GenBank Nucleotide #: NM_000632.3
• UniProt Accession #: P11215; Q4VAK0; Q4VAK1; Q4VAK2
• Molecular Weight: 127,307 Da
• NCBI Official Full Name: integrin alpha-M isoform 2
• NCBI Official Synonym Full Names: integrin, alpha M (complement component 3 receptor 3 subunit)
• NCBI Official Symbol: ITGAM
• NCBI Official Synonym Symbols: CR3A; MO1A; CD11B; MAC-1; MAC1A; SLEB6
• NCBI Protein Information: integrin alpha-M
• UniProt Protein Name: Integrin alpha-M
• Protein Family: Integrin
• UniProt Gene Name: ITGAM
• UniProt Synonym Gene Names: CD11B; CR3A
• UniProt Entry Name: ITAM_HUMAN

NCBI Description

This gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

Uniprot Description

ITGAM: a single-pass type I membrane protein of the integrin alpha chain family. Implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. Also known as CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the RGD peptide in C3b. A receptor for fibrinogen, factor X and ICAM1. Heterodimer of an alpha and a beta subunit. Alpha-M associates with beta-2. Predominantly expressed in monocytes and granulocytes.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Cell adhesion

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: cell surface; plasma membrane; nucleus; integrin complex; external side of plasma membrane

Molecular Function: heparan sulfate proteoglycan binding; heparin binding; protein binding; opsonin binding; protein heterodimerization activity; metal ion binding; glycoprotein binding

Biological Process: integrin-mediated signaling pathway; neutrophil chemotaxis; extracellular matrix organization and biogenesis; microglia development; cellular extravasation; toll-like receptor signaling pathway; activated T cell proliferation; innate immune response; blood coagulation; cell adhesion; toll-like receptor 4 signaling pathway; leukocyte migration; leukocyte migration during inflammatory response

Disease: Systemic Lupus Erythematosus, Susceptibility To, 6