Western Blot (WB)
(Western blot analysis of TIF1 alpha expression in MCF7 (A), HeLa (B) whole cell lysates.)
Rabbit TIF1 alpha Polyclonal Antibody | anti-TRIM24 antibody
Anti-TIF1 alpha Antibody
Western Blot (WB)
(Western blot analysis of TIF1 alpha expression in MCF7 (A), HeLa (B) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of TIF1 alpha expression in MCF7 (A), HeLa (B) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of TIF1 alpha staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of TIF1 alpha staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene mediates transcriptional control by interaction with the activation function 2 (AF2) region of several nuclear receptors, including the estrogen, retinoic acid, and vitamin D3 receptors. The protein localizes to nuclear bodies and is thought to associate with chromatin and heterochromatin-associated factors. The protein is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains - a RING, a B-box type 1 and a B-box type 2 - and a coiled-coil region. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
TRIM24: an atypical protein kinase that functions as a transcriptional coactivator. Originally identified as a mammalian protein that enhances the AF-2 activity of the retinoid X receptor (RXR) in a yeast genetic screen. Binds to nuclear receptors in an agonist and AF2-activating domain (AF2-AD) dependent manner. Binding to the agonist-nuclear receptor complex induces its own hyperphosphorylation as well as the phosphorylation of TFIIEa, TAFII28, and TAFII55. Belongs to a family of nuclear proteins that includes TIF1b and TIF1g. TIF1 proteins are characterized by an N-terminal region that contains a RING finger-B boxes-coiled coil (RBCC) motif, a poorly conserved central region, a C-terminal region that contains a PHD finger, and a bromodomain. Interacts with the heterochromatin-associated proteins HP1a, MOD1 (HP1b), and MOD2 (HP1g) and with the transcriptional repression domain KRAB, which is present in many Kruppel-type (C2H2) zinc finger proteins. May act as a repressor through the formation of transcriptionally inactive heterochromatin. Two splice variant isoforms have been described.
Protein type: Nuclear receptor co-regulator; Kinase, protein; EC 6.3.2.-; Protein kinase, atypical; Ubiquitin conjugating system; Tumor suppressor; Transcription, coactivator/corepressor; ATYPICAL group; TIF1 family
Chromosomal Location of Human Ortholog: 7q32-q34
Cellular Component: nucleoplasm; perichromatin fibrils; cytosol; nucleus
Molecular Function: ligand-dependent nuclear receptor binding; zinc ion binding; p53 binding; transcription coactivator activity; ubiquitin-protein ligase activity; estrogen response element binding; methylated histone residue binding; protein kinase activity; protein binding; sequence-specific DNA binding; chromatin binding; ligase activity; receptor binding
Biological Process: regulation of apoptosis; calcium ion homeostasis; transcription from RNA polymerase II promoter; response to peptide hormone stimulus; negative regulation of cell proliferation; protein amino acid autophosphorylation; positive regulation of transcription, DNA-dependent; protein ubiquitination; regulation of protein stability; protein catabolic process; negative regulation of transcription, DNA-dependent
Disease: Thyroid Carcinoma, Papillary
Research Articles on TRIM24
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Product Notes
The TRIM24 trim24 (Catalog #AAA820854) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-TIF1 alpha Antibody reacts with Human, Bovine, Dog, Monkey, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's TIF1 alpha can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB (1/500 - 1/1000), IH (1/100 - 1/200). Researchers should empirically determine the suitability of the TRIM24 trim24 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TIF1 alpha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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