UBA3 sirna

UBA3 siRNA (Mouse)

Cat. Num. AAA8208497

• Gene Names: Uba3; Ube1c; AI256736; AI848246; AW546539; A830034N06Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: UBA3; UBA3 siRNA (Mouse); UBE1C; NEDD8-activating enzyme E1 catalytic subunit; NEDD8-activating enzyme E1C; Ubiquitin-activating enzyme E1C; Ubiquitin-like modifier-activating enzyme 3; Ubiquitin-activating enzyme 3; UBA3 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: UBA3 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 448

• Applicable Applications for UBA3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse UBA3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for UBA3 sirna

siRNA to inhibit UBA3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 162287057
• NCBI GeneID: 22200
• NCBI Accession #: NP_001104576.1
• NCBI GenBank Nucleotide #: NM_001111106.2
• UniProt Accession #: Q8C878; O88598; Q3TG68; Q9D6B7
• Molecular Weight: 44,326 Da
• NCBI Official Full Name: NEDD8-activating enzyme E1 catalytic subunit isoform 2
• NCBI Official Synonym Full Names: ubiquitin-like modifier activating enzyme 3
• NCBI Official Symbol: Uba3
• NCBI Official Synonym Symbols: Ube1c; AI256736; AI848246; AW546539; A830034N06Rik
• NCBI Protein Information: NEDD8-activating enzyme E1 catalytic subunit
• UniProt Protein Name: NEDD8-activating enzyme E1 catalytic subunit
• Protein Family: Uba3-binding protein
• UniProt Gene Name: Uba3
• UniProt Synonym Gene Names: Ube1c; Ubiquitin-activating enzyme 3
• UniProt Entry Name: UBA3_MOUSE

NCBI Description

The protein encoded by this gene is the catalytic subunit of the enzyme that activates NEDD8, a ubiquitin-like molecule that binds to its target proteins through an enzymatic reaction analagous to ubiquitylation. Embryonic mice deficient for this protein die prior to implantation and display apoptosis of the inner cell mass. Trophoblastic cells cannot enter S phase, demonstrating that this gene is required for cell cycle progression during embryogenesis. Two pseudogenes have been found for this gene. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2014]

Uniprot Description

UBA3: Catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Down-regulates steroid receptor activity. Necessary for cell cycle progression. Belongs to the ubiquitin-activating E1 family. UBA3 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 6.3.2.-; Cell cycle regulation; Nuclear receptor co-regulator; EC 6.3.2.19; Ubiquitin ligase; Ubiquitin conjugating system; Ligase

Cellular Component: cytosol; nucleus

Molecular Function: NEDD8 activating enzyme activity; ligand-dependent nuclear receptor binding; small protein activating enzyme activity; coenzyme F420-2 alpha-glutamyl ligase activity; ribosomal S6-glutamic acid ligase activity; nucleotide binding; protein binding; acid-amino acid ligase activity; protein heterodimerization activity; UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity; coenzyme F420-0 gamma-glutamyl ligase activity; ATP binding; ligase activity

Biological Process: protein neddylation; regulation of cell cycle; protein modification process; mitotic cell cycle; negative regulation of transcription, DNA-dependent; endomitotic cell cycle; cell cycle