KRT8 sirna

KRT8 siRNA (Rat)

Cat. Num. AAA8208061

• Gene Names: Krt8; CYKER; Krt2-8
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: KRT8; KRT8 siRNA (Rat); KRT2-8; Keratin type II cytoskeletal 8; Cytokeratin endo A; Cytokeratin-8; CK-8; Keratin-8; K8; Type-II keratin Kb8; KRT8 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: KRT8 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 483

• Applicable Applications for KRT8 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat KRT8 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for KRT8 sirna

siRNA to inhibit KRT8 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 40786432
• NCBI GeneID: 25626
• NCBI Accession #: NP_955402.1
• NCBI GenBank Nucleotide #: NM_199370.1
• UniProt Accession #: Q10758; Q5WPB3
• Molecular Weight: 54,019 Da
• NCBI Official Full Name: keratin, type II cytoskeletal 8
• NCBI Official Synonym Full Names: keratin 8, type II
• NCBI Official Symbol: Krt8
• NCBI Official Synonym Symbols: CYKER; Krt2-8
• NCBI Protein Information: keratin, type II cytoskeletal 8
• UniProt Protein Name: Keratin, type II cytoskeletal 8
• Protein Family: Keratin
• UniProt Gene Name: Krt8
• UniProt Synonym Gene Names: Krt2-8; CK-8; K8
• UniProt Entry Name: K2C8_RAT

NCBI Description

an intermediate filament protein; may play a role in prostrate cells [RGD, Feb 2006]

Uniprot Description

K8: a type II cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Phosphorylation of keratins at specific sites affects their organization, assembly dynamics, and their interaction with signaling molecules. Phsophorylated by p38 kinase, regulating cellular keratin filament reorganization. Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Mutation of this protein is a risk factor for cryptogenic liver failure.

Protein type: Cytoskeletal

Cellular Component: dystrophin-associated glycoprotein complex; nucleoplasm; intermediate filament cytoskeleton; nuclear matrix; costamere; cytoplasm; keratin filament; intermediate filament; intercellular junction; nucleus; Z disc; sarcolemma

Molecular Function: protein binding; protein complex binding; structural molecule activity

Biological Process: tumor necrosis factor-mediated signaling pathway; sarcomere organization; response to other organism; response to hydrostatic pressure