MEIS2 sirna

MEIS2 siRNA (Human)

Cat. Num. AAA8207953

• Gene Names: MEIS2; MRG1; HsT18361
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MEIS2; MEIS2 siRNA (Human); MRG1; Homeobox protein Meis2; Meis1-related protein 1; MEIS2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: MEIS2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 388

• Applicable Applications for MEIS2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human MEIS2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MEIS2 sirna

siRNA to inhibit MEIS2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 333805648
• NCBI GeneID: 4212
• NCBI Accession #: NP_001207411.1
• NCBI GenBank Nucleotide #: NM_001220482.1
• UniProt Accession #: O14770; Q96DI2; Q96KI4; Q96KI5; Q9NRS1; Q9NRS2; Q9NRS3; A6NJI5; A8MWD5; B3KP98; B3KPQ6
• Molecular Weight: 42,272 Da
• NCBI Official Full Name: homeobox protein Meis2 isoform d
• NCBI Official Synonym Full Names: Meis homeobox 2
• NCBI Official Symbol: MEIS2
• NCBI Official Synonym Symbols: MRG1; HsT18361
• NCBI Protein Information: homeobox protein Meis2
• UniProt Protein Name: Homeobox protein Meis2
• Protein Family: Homeobox protein
• UniProt Gene Name: MEIS2
• UniProt Synonym Gene Names: MRG1
• UniProt Entry Name: MEIS2_HUMAN

NCBI Description

This gene encodes a homeobox protein belonging to the TALE ('three amino acid loop extension') family of homeodomain-containing proteins. TALE homeobox proteins are highly conserved transcription regulators, and several members have been shown to be essential contributors to developmental programs. Multiple transcript variants encoding distinct isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

MEIS2: a homeobox protein belonging to the TALE ('three amino acid loop extension') family of homeodomain-containing proteins. TALE homeobox proteins are highly conserved transcription regulators, and several members have been shown to be essential contributors to developmental programs. Multiple transcript variants encoding distinct isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 15q14

Cellular Component: nucleus

Molecular Function: protein binding; sequence-specific DNA binding; transcription cofactor activity; transcription corepressor activity; transcription factor binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; negative regulation of myeloid cell differentiation; eye development; response to mechanical stimulus; pancreas development; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter