Mouse anti-Human CEACAM-1 Monoclonal Antibody | anti-CEACAM-1 antibody

Mouse Anti-Human CEACAM-1

Cat. Num. AAA690824

• Gene Names: CEACAM1; BGP; BGP1; BGPI
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, ELISA, Immunofluorescence, Immunoprecipitation, Flow Cytometry, Functional Assay
• Purity: Protein G purified
• Synonyms: CEACAM-1; Monoclonal Antibody; Mouse Anti-Human CEACAM-1; Anti-human CEACAM-1 (#B3-17); Carcinoembryonic antigen-related cell adhesion molecule 1; anti-CEACAM-1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: B3-17
• Specificity: Anti-human CEACAM-1 (B3) interacts solely with human CEACAM-1 and shows no cross-reactivity with other CEACAMs expressed in human or other species! B3 binds to the A1-B-Domain!
• Purity/Purification: Protein G purified
• Form/Format: Lyophilized
• Sequence Length: 464

• Applicable Applications for anti-CEACAM-1 antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
• Application Notes: ELISA: Use at 10 ug/ml FACS: Use at 10 ug/ml IF/IHC Use at 10 ug/ml (weak) IP 10 ug/IP Western Blot: Use at 10 ug/ml
• Immunogen: Recombinant human sCEACAM-1/Fc lacking the N-domain (HEK293)
• Preparation: Monoclonal antibodies were produced with the help of NS1/0 mice using recombinant human soluble CEACAM-1-Fc lacking the N-domain (produced in HEK293 cells) as the immunizing antigen.
• Buffer: PBS
• Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
• Preparation and Storage: The lyophilized antibody is stable at room temperature for up to 1 month. The reconstituted antibody is stable for at least two weeks at 2-8 degree C. Frozen aliquots are stable for at least 6 months when stored at -20 degree C.

Testing Data

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Related Product Information for anti-CEACAM-1 antibody

Carcinoembryonic Antigen-related Cell Adhesion Molecule 1 (CEACAM-1), also known as CD66a and BGP-I, is a 115 kDa type I transmembrane glycoprotein that belongs to the membrane-bound CEA subfamily of the CEA superfamily. On the surface of cells, it forms noncovalent homo- and heterodimers. The extracellular region contains three C2-type Ig-like domains and one N-terminal V-type Ig-like domain. Multiple splice variants involving regions C-terminal to the second C2-type domain (aa 320 and beyond) exist.

References

1. Muturi HT et al, PLOS/one Vol 8, Issue 9 (2013) 2. Klaile E et al, Respiratory Research 14 (2013) 3. Waggener and Ergun, Exp Cell Res 261 (2000) 4. Barnett et al, J Cell Biol 108 (1989) 5. Hinoda et al, Proc Natl Acad Sci 85 (1998) 6. Müller et al, Blood 105 (2006) 7. Kilic et al, Blood 110 (2007) 8. Zengin et al, Development 133 (2006) 9. Oliveira-Ferrer et al, Cancer Res 64 (2004);

NCBI and Uniprot Product Information

• NCBI GI #: 68161541
• NCBI GeneID: 634
• NCBI Accession #: NP_001192273.1
• NCBI GenBank Nucleotide #: NM_001205344.1
• UniProt Accession #: P13688; O60430; Q069I7; Q13854; Q13857; Q13858; Q13859; Q13860; Q15600; Q15601; A6NE38; A8MY49
• Molecular Weight: 57,560 Da
• NCBI Official Full Name: carcinoembryonic antigen-related cell adhesion molecule 1 isoform 2
• NCBI Official Synonym Full Names: carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein)
• NCBI Official Symbol: CEACAM1
• NCBI Official Synonym Symbols: BGP; BGP1; BGPI
• NCBI Protein Information: carcinoembryonic antigen-related cell adhesion molecule 1; antigen CD66; CD66a antigen
• UniProt Protein Name: Carcinoembryonic antigen-related cell adhesion molecule 1
• UniProt Gene Name: CEACAM1
• UniProt Synonym Gene Names: BGP; BGP1; BGP-1
• UniProt Entry Name: CEAM1_HUMAN

NCBI Description

This gene encodes a member of the carcinoembryonic antigen (CEA) gene family, which belongs to the immunoglobulin superfamily. Two subgroups of the CEA family, the CEA cell adhesion molecules and the pregnancy-specific glycoproteins, are located within a 1.2 Mb cluster on the long arm of chromosome 19. Eleven pseudogenes of the CEA cell adhesion molecule subgroup are also found in the cluster. The encoded protein was originally described in bile ducts of liver as biliary glycoprotein. Subsequently, it was found to be a cell-cell adhesion molecule detected on leukocytes, epithelia, and endothelia. The encoded protein mediates cell adhesion via homophilic as well as heterophilic binding to other proteins of the subgroup. Multiple cellular activities have been attributed to the encoded protein, including roles in the differentiation and arrangement of tissue three-dimensional structure, angiogenesis, apoptosis, tumor suppression, metastasis, and the modulation of innate and adaptive immune responses. Multiple transcript variants encoding different isoforms have been reported, but the full-length nature of all variants has not been defined. [provided by RefSeq, May 2010]

Uniprot Description

Subcellular location: Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted Ref.4 Ref.6. Isoform 3: Secreted Ref.4 Ref.6. Isoform 4: Secreted Ref.4 Ref.6. Isoform 5: Cell membrane; Single-pass type I membrane protein. Isoform 6: Cell membrane; Single-pass type I membrane protein. Isoform 7: Cell membrane; Single-pass type I membrane protein. Isoform 8: Cell membrane; Single-pass type I membrane protein. Note: Localizes to sites of cell-cell contact. Ref.4 Ref.6

Sequence similarities: Belongs to the immunoglobulin superfamily. CEA family.Contains 3 Ig-like C2-type (immunoglobulin-like) domains.Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Sequence caution: The sequence AAA57141.1 differs from that shown. Reason: Erroneous gene model prediction.