CFTR sirna

CFTR siRNA (Mouse)

Cat. Num. AAA8207850

• Gene Names: Cftr; Abcc7; AW495489
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CFTR; CFTR siRNA (Mouse); ABCC7; Cystic fibrosis transmembrane conductance regulator; ATP-binding cassette sub-family C member 7; Channel conductance-controlling ATPase; cAMP-dependent chloride channel; CFTR sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: CFTR siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 600

• Applicable Applications for CFTR sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CFTR gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CFTR sirna

siRNA to inhibit CFTR expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 14141185
• NCBI GeneID: 12638
• NCBI Accession #: NP_066388.1
• NCBI GenBank Nucleotide #: NM_021050.2
• UniProt Accession #: P26361; Q63893; Q63894; Q9JKQ6
• Molecular Weight: 68,782 Da
• NCBI Official Full Name: cystic fibrosis transmembrane conductance regulator
• NCBI Official Synonym Full Names: cystic fibrosis transmembrane conductance regulator
• NCBI Official Symbol: Cftr
• NCBI Official Synonym Symbols: Abcc7; AW495489
• NCBI Protein Information: cystic fibrosis transmembrane conductance regulator
• UniProt Protein Name: Cystic fibrosis transmembrane conductance regulator
• Protein Family: Cystic fibrosis transmembrane conductance regulator
• UniProt Gene Name: Cftr
• UniProt Synonym Gene Names: Abcc7; CFTR
• UniProt Entry Name: CFTR_MOUSE

NCBI Description

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This gene encodes the cystic fibrosis transmembrane regulator and a chloride channel that controls the regulation of other transport pathways. Mutations in this gene have been associated with autosomal recessive disorders such as cystic fibrosis and congenital bilateral aplasia of the vas deferens. Alternative splicing of exons 4, 5, and 11 have been observed, but full-length transcripts have not yet been fully described. [provided by RefSeq, Jul 2008]

Uniprot Description

CFTR: a member of the MRP subfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. Functions as a chloride channel and controls the regulation of other transport pathways. Mutations have been observed in patients with the autosomal recessive disorders cystic fibrosis (CF) and congenital bilateral aplasia of the vas deferens (CBAVD). Alternative splice

Protein type: Membrane protein, integral; Channel, chloride; EC 3.6.3.49; Membrane protein, multi-pass; Transporter; Hydrolase; Transporter, ABC family

Cellular Component: microvillus; cytoplasmic vesicle membrane; cell surface; protein complex; cell; basolateral plasma membrane; early endosome; integral to membrane; recycling endosome; membrane; apical plasma membrane; cytoplasm; plasma membrane; intracellular; endosome

Molecular Function: bicarbonate transmembrane transporter activity; chloride channel activity; hydrolase activity; ATPase activity; nucleotide binding; PDZ domain binding; protein binding; chloride channel inhibitor activity; enzyme binding; ATPase activity, coupled to transmembrane movement of substances; chloride transmembrane transporter activity; channel-conductance-controlling ATPase activity; ATP binding

Biological Process: intracellular pH elevation; cholesterol transport; iodide transport; water transport; transepithelial chloride transport; membrane hyperpolarization; chloride transport; positive regulation of vasodilation; cholesterol biosynthetic process; vasodilation; bicarbonate transport; transport; ion transport; transmembrane transport; sperm capacitation; lung development