Mouse anti-Human RCAS1/Estrogen Receptor Binding Site Associated, Antigen 9 Monoclonal Antibody | anti-EBAG9 antibody

RCAS1/Estrogen Receptor Binding Site Associated, Antigen 9

Cat. Num. AAA4381383

• Gene Names: EBAG9; EB9; PDAF
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: RCAS1/Estrogen Receptor Binding Site Associated; Antigen 9; Monoclonal Antibody; BAG9; cancer associated surface antigen; cancer associated surface antigen RCAS1; EB9; estrogen receptor binding fragment associated gene 9; PDAF; receptor binding cancer antigen expressed on SiSo cells; anti-EBAG9 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a, kappa
• Clone Number: CPTC-EBAG9-2
• Specificity: EBAG9, also known as RCAS1, is an estrogen-transcribed protein. Soluble and membranous RCAS1 proteins may play a role in the immune escape of tumor cells by promoting T lymphocyte inhibition of growth and apoptosis. RCAS1 is expressed in a wide variety of cancers, including uterine, ovarian, and lung cancer cells, and acts as a ligand for a putative receptor present on peripheral lymphocytes. RCAS1 is highly expressed not only in cancer cells but also in non-tumor bile duct cells subject to immune attack. RCAS1 inhibits the in vitro growth of receptor-expressing cells and induces apoptosis, contributing to the ability of tumor cells to evade host immune surveillance. High expression of RCAS1 significantly correlates with tumor progression and with poor outcome for many cancer patients.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 213

• Applicable Applications for anti-EBAG9 antibody: Immunohistochemistry (IHC) Paraffin
• Application Notes: Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Positive Control: MCF-7, Jurkat; 293 cell lysates; Human skeletal muscle tissue.
• Immunogen: Recombinant full-length human EBAG9 protein
• Cellular Localization: Golgi apparatus.
• Hu-chromosome Location: 8q23.2
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Tonsil stained with RCAS1 Mouse Monoclonal Antibody (CPTC-EBAG9-2).)

SDS-Page

(SDS-PAGE Analysis Purified RCAS1 Mouse Monoclonal Antibody (CPTC-EBAG9-2). Confirmation of Purity and Integrity of Antibody.)

References

1. Ruder, C. et al. (2009) J Clin Invest 119, 2184-203. 2. Watanabe, T. et al. (1998) Mol Cell Biol 18, 442-9.

NCBI and Uniprot Product Information

• NCBI GI #: 8928283
• NCBI GeneID: 9166
• UniProt Accession #: O00559
• Molecular Weight: 32kDa
• NCBI Official Full Name: Receptor-binding cancer antigen expressed on SiSo cells
• NCBI Official Synonym Full Names: estrogen receptor binding site associated antigen 9
• NCBI Official Symbol: EBAG9
• NCBI Official Synonym Symbols: EB9; PDAF
• NCBI Protein Information: receptor-binding cancer antigen expressed on SiSo cells
• UniProt Protein Name: Receptor-binding cancer antigen expressed on SiSo cells
• Protein Family: Receptor-binding cancer antigen
• UniProt Gene Name: EBAG9
• UniProt Synonym Gene Names: RCAS1
• UniProt Entry Name: RCAS1_HUMAN

NCBI Description

This gene was identified as an estrogen-responsive gene. Regulation of transcription by estrogen is mediated by estrogen receptor, which binds to the estrogen-responsive element found in the 5'-flanking region of this gene. The encoded protein is a tumor-associated antigen that is expressed at high frequency in a variety of cancers. Alternate splicing results in multiple transcript variants. A pseudogene of this gene has been defined on chromosome 10. [provided by RefSeq, Jul 2013]

Uniprot Description

RCAS1: May participate in suppression of cell proliferation and induces apoptotic cell death through activation of interleukin-1- beta converting enzyme (ICE)-like proteases. Homodimer. By estrogen. Widely expressed. Expressed in ovary, testis, prostate, thymus, muscle and heart, but not in small intestine, colon, lymph nodes, or peripherical blood lymphocytes. The protein is not detected in any of the above organs.

Protein type: Membrane protein, integral; Apoptosis

Chromosomal Location of Human Ortholog: 8q23

Cellular Component: Golgi membrane; Golgi apparatus; focal adhesion; plasma membrane; integral to membrane; secretory granule

Molecular Function: apoptotic protease activator activity

Biological Process: apoptosis; regulation of cell growth