SRP72 sirna

SRP72 siRNA (Human)

Cat. Num. AAA8207819

• Gene Names: SRP72; BMFF; BMFS1; HEL103
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SRP72; SRP72 siRNA (Human); Signal recognition particle subunit SRP72; Signal recognition particle 72 kDa protein; SRP72 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SRP72 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 610

• Applicable Applications for SRP72 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SRP72 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SRP72 sirna

siRNA to inhibit SRP72 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 391353390
• NCBI GeneID: 6731
• NCBI Accession #: NP_001254651.1
• NCBI GenBank Nucleotide #: NM_001267722.1
• UniProt Accession #: O76094; Q7Z3C0; G5E9Z8
• Molecular Weight: 67,879 Da
• NCBI Official Full Name: signal recognition particle subunit SRP72 isoform 2
• NCBI Official Synonym Full Names: signal recognition particle 72kDa
• NCBI Official Symbol: SRP72
• NCBI Official Synonym Symbols: BMFF; BMFS1; HEL103
• NCBI Protein Information: signal recognition particle subunit SRP72
• UniProt Protein Name: Signal recognition particle subunit SRP72
• Protein Family: Signal recognition particle
• UniProt Gene Name: SRP72
• UniProt Synonym Gene Names: SRP72
• UniProt Entry Name: SRP72_HUMAN

NCBI Description

This gene encodes the 72 kDa subunit of the signal recognition particle (SRP), a ribonucleoprotein complex that mediates the targeting of secretory proteins to the endoplasmic reticulum (ER). The SRP complex consists of a 7S RNA and 6 protein subunits: SRP9, SRP14, SRP19, SRP54, SRP68, and SRP72, that are bound to the 7S RNA as monomers or heterodimers. SRP has at least 3 distinct functions that can be associated with the protein subunits: signal recognition, translational arrest, and ER membrane targeting by interaction with the docking protein. Mutations in this gene are associated with familial bone marrow failure. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2012]

Uniprot Description

SRP72: Signal-recognition-particle assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum membrane. Binds the 7S RNA only in presence of SRP68. This ribonucleoprotein complex might interact directly with the docking protein in the ER membrane and possibly participate in the elongation arrest function. Defects in SRP72 are the cause of bone marrow failure, familial (BMFF). BMFF is an autosomal dominant disease characterized by aplastic anemia and myelodysplasia resulting from bone marrow failure. Aplastic anemia is a form of anemia in which the bone marrow fails to produce adequate numbers of peripheral blood elements. Myelodysplasia is a clonal hematopoietic stem cell disorder in which immature cells in the bone marrow become malformed and dysfunctional. Belongs to the SRP72 family.

Protein type: Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 4q11

Cellular Component: endoplasmic reticulum; cytoplasm; nucleolus; plasma membrane; signal recognition particle, endoplasmic reticulum targeting; cytosol

Molecular Function: signal recognition particle binding; 7S RNA binding

Biological Process: response to drug; SRP-dependent cotranslational protein targeting to membrane; cellular protein metabolic process; translation; gene expression

Disease: Bone Marrow Failure Syndrome 1