MYH3 sirna

MYH3 siRNA (Rat)

Cat. Num. AAA8207815

• Gene Names: Myh3; Myhse; RNMHCG
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MYH3; MYH3 siRNA (Rat); Myosin-3; Myosin heavy chain 3; Myosin heavy chain. fast skeletal muscle. embryonic; MYH3 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: MYH3 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1,940

• Applicable Applications for MYH3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat MYH3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MYH3 sirna

siRNA to inhibit MYH3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6981234
• NCBI GeneID: 24583
• NCBI Accession #: NP_036736.1
• NCBI GenBank Nucleotide #: NM_012604.1
• UniProt Accession #: P12847
• Molecular Weight: 223,858 Da
• NCBI Official Full Name: myosin-3
• NCBI Official Synonym Full Names: myosin, heavy chain 3, skeletal muscle, embryonic
• NCBI Official Symbol: Myh3
• NCBI Official Synonym Symbols: Myhse; RNMHCG
• NCBI Protein Information: myosin-3
• UniProt Protein Name: Myosin-3
• UniProt Gene Name: Myh3
• UniProt Entry Name: MYH3_RAT

NCBI Description

heavy chain of myosin; involved in muscle contraction [RGD, Feb 2006]

Uniprot Description

MYH3: Muscle contraction. Defects in MYH3 are the cause of distal arthrogryposis type 2A (DA2A); also known as Freeman-Sheldon syndrome (FSS). Distal arthrogryposis is a clinically and genetically heterogeneous group of disorders characterized by bone anomalies and joint contractures of the hands and feet, causing medially overlapping fingers, clenched fists, ulnar deviation of fingers, camptodactyly and positional foot deformities. It is a disorder of primary limb malformation without primary neurologic or muscle disease. DA2A is the most severe form of distal arthrogryposis. Affected individuals have contractures of the orofacial muscles, characterized by microstomia with pouting lips, H-shaped dimpling of the chin, deep nasolabial folds, and blepharophimosis. Dysphagia, failure to thrive, growth deficit, and life-threatening respiratory complications (caused by structural anomalies of the oropharynx and upper airways) are frequent. Inheritance is autosomal dominant. Defects in MYH3 are the cause of distal arthrogryposis type 2B (DA2B); also known as Sheldon-Hall syndrome (SHS) or arthrogryposis multiplex congenita distal type 2B (AMCD2B). DA2B is a form of inherited multiple congenital contractures. Affected individuals have vertical talus, ulnar deviation in the hands, severe camptodactyly, and a distinctive face characterized by a triangular shape, prominent nasolabial folds, small mouth and a prominent chin. DA2B is the most common of the distal arthrogryposis syndromes. It is similar to DA2A but the facial contractures are less dramatic.

Protein type: Motility/polarity/chemotaxis; Motor

Cellular Component: myofibril; contractile fiber; myosin complex

Molecular Function: actin filament binding; calmodulin binding; microfilament motor activity; motor activity; actin binding; ATP binding

Biological Process: skeletal muscle contraction; metabolic process