Rabbit anti-Human DIO2 Polyclonal Antibody | anti-DIO2 antibody

DIO2, ID (Type II iodothyronine deiodinase, Type-II 5'-deiodinase, Type 2 DI, DIOII, 5DII, TXDI2, ITDI2, DIO2) (Azide free) (HRP)

Cat. Num. AAA6472464

• Gene Names: DIO2; D2; 5DII; SelY; DIOII; TXDI2
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: DIO2; Polyclonal Antibody; ID (Type II iodothyronine deiodinase; Type-II 5'-deiodinase; Type 2 DI; DIOII; 5DII; TXDI2; ITDI2; DIO2) (Azide free) (HRP); anti-DIO2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human DIO2.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-DIO2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-1:500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human DIO2 (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of DIO2 Antibody (Center) in MCF-7 cell line lysates (35ug/lane). DIO2 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(DIO2 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded mouse brain followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the mouse DIO2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(DIO2 Antibody (Center) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-DIO2 antibody

Antibodies; Enzymes

References

1. He, B., et.al., Prog. Neuropsychopharmacol. Biol. Psychiatry 33(6), 986-990 (2009). 2. Heemstra, K.A., et.al., J. Clin. Endocrinol. Metab. 94(6), 2144-2150 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 7549805
• NCBI GeneID: 1734
• NCBI Accession #: NP_000784.2; NP_054644.1; NP_001007024.1
• NCBI GenBank Nucleotide #: NM_000793.5
• UniProt Accession #: Q92813; Q6B0A3; Q9HCP8; Q9P1W4; Q9UDZ1; B9EGK0; G3V315
• Molecular Weight: 34,704 Da
• NCBI Official Full Name: type II iodothyronine deiodinase isoform a
• NCBI Official Synonym Full Names: deiodinase, iodothyronine, type II
• NCBI Official Symbol: DIO2
• NCBI Official Synonym Symbols: D2; 5DII; SelY; DIOII; TXDI2
• NCBI Protein Information: type II iodothyronine deiodinase
• UniProt Protein Name: Type II iodothyronine deiodinase
• Protein Family: Type II iodothyronine deiodinase
• UniProt Gene Name: DIO2
• UniProt Synonym Gene Names: ITDI2; TXDI2

NCBI Description

The protein encoded by this gene belongs to the iodothyronine deiodinase family. It catalyzes the conversion of prohormone thyroxine (3,5,3',5'-tetraiodothyronine, T4) to the bioactive thyroid hormone (3,5,3'-triiodothyronine, T3) by outer ring 5'-deiodination. This gene is widely expressed, including in thyroid, placenta, pituitary and brain. It is thought to be responsible for the 'local' production of T3, and thus important in influencing thyroid hormone action in these tissues. It has also been reported to be highly expressed in thyroids of patients with Graves disease, and in follicular adenomas. The intrathyroidal T4 to T3 conversion by this enzyme may contribute significantly to the relative increase in thyroidal T3 production in these patients. This protein is a selenoprotein containing the rare selenocysteine (Sec) amino acid at its active site, and may contain additional Sec residues. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2016]

Uniprot Description

Responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into T3 (3,5,3'-triiodothyronine). Essential for providing the brain with appropriate levels of T3 during the critical period of development.