SPEN sirna

SPEN siRNA (Human)

Cat. Num. AAA8207632

• Gene Names: SPEN; MINT; SHARP; RBM15C; HIAA0929
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SPEN; SPEN siRNA (Human); KIAA0929; MINT; SHARP; Msx2-interacting protein; SMART/HDAC1-associated repressor protein; SPEN homolog; SPEN sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SPEN siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 3,664

• Applicable Applications for SPEN sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SPEN gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SPEN sirna

siRNA to inhibit SPEN expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 14790190
• NCBI GeneID: 23013
• NCBI Accession #: NP_055816.2
• NCBI GenBank Nucleotide #: NM_015001.2
• UniProt Accession #: Q96T58; Q9H9A8; Q9NWH5; Q9UQ01; Q9Y556
• Molecular Weight: 402,248 Da
• NCBI Official Full Name: msx2-interacting protein
• NCBI Official Synonym Full Names: spen family transcriptional repressor
• NCBI Official Symbol: SPEN
• NCBI Official Synonym Symbols: MINT; SHARP; RBM15C; HIAA0929
• NCBI Protein Information: msx2-interacting protein
• UniProt Protein Name: Msx2-interacting protein
• Protein Family: Protein split ends
• UniProt Gene Name: SPEN
• UniProt Synonym Gene Names: KIAA0929; MINT; SHARP
• UniProt Entry Name: MINT_HUMAN

NCBI Description

This gene encodes a hormone inducible transcriptional repressor. Repression of transcription by this gene product can occur through interactions with other repressors, by the recruitment of proteins involved in histone deacetylation, or through sequestration of transcriptional activators. The product of this gene contains a carboxy-terminal domain that permits binding to other corepressor proteins. This domain also permits interaction with members of the NuRD complex, a nucleosome remodeling protein complex that contains deacetylase activity. In addition, this repressor contains several RNA recognition motifs that confer binding to a steroid receptor RNA coactivator; this binding can modulate the activity of both liganded and nonliganded steroid receptors. [provided by RefSeq, Jul 2008]

Uniprot Description

SHARP: May serve as a nuclear matrix platform that organizes and integrates transcriptional responses. In osteoblasts, supports transcription activation: synergizes with RUNX2 to enhance FGFR2- mediated activation of the osteocalcin FGF-responsive element (OCFRE). Has also been shown to be an essential corepressor protein, which probably regulates different key pathways such as the Notch pathway. Negative regulator of the Notch pathway via its interaction with RBPSUH, which prevents the association between NOTCH1 and RBPSUH, and therefore suppresses the transactivation activity of Notch signaling. Blocks the differentiation of precursor B-cells into marginal zone B-cells. Probably represses transcription via the recruitment of large complexes containing histone deacetylase proteins. May bind both to DNA and RNA. Belongs to the RRM Spen family.

Protein type: Transcription factor; Spliceosome; Nuclear receptor co-regulator; RNA-binding

Chromosomal Location of Human Ortholog: 1p36

Cellular Component: nucleoplasm; transcriptional repressor complex

Molecular Function: protein binding; nucleotide binding; transcription corepressor activity; transcription factor activity; single-stranded DNA binding

Biological Process: Notch signaling pathway; positive regulation of neurogenesis; transcription, DNA-dependent; viral reproduction; positive regulation of transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent