G6PC2 sirna

G6PC2 siRNA (Human)

Cat. Num. AAA8207430

• Gene Names: G6PC2; IGRP
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: G6PC2; G6PC2 siRNA (Human); IGRP; Glucose-6-phosphatase 2; G-6-Pase 2; G6Pase 2; Islet-specific glucose-6-phosphatase catalytic subunit-related protein; G6PC2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: G6PC2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 154

• Applicable Applications for G6PC2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human G6PC2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for G6PC2 sirna

siRNA to inhibit G6PC2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 126273546
• NCBI GeneID: 57818
• NCBI Accession #: NP_001075155.1
• NCBI GenBank Nucleotide #: NM_001081686.1
• UniProt Accession #: Q9NQR9; Q6AHZ0; E9PAX2
• Molecular Weight: 17,757 Da
• NCBI Official Full Name: glucose-6-phosphatase 2 isoform 2
• NCBI Official Synonym Full Names: glucose-6-phosphatase, catalytic, 2
• NCBI Official Symbol: G6PC2
• NCBI Official Synonym Symbols: IGRP
• NCBI Protein Information: glucose-6-phosphatase 2
• UniProt Protein Name: Glucose-6-phosphatase 2
• Protein Family: Glucose-6-phosphatase
• UniProt Gene Name: G6PC2
• UniProt Synonym Gene Names: IGRP; G-6-Pase 2; G6Pase 2
• UniProt Entry Name: G6PC2_HUMAN

NCBI Description

This gene encodes an enzyme belonging to the glucose-6-phosphatase catalytic subunit family. These enzymes are part of a multicomponent integral membrane system that catalyzes the hydrolysis of glucose-6-phosphate, the terminal step in gluconeogenic and glycogenolytic pathways, allowing the release of glucose into the bloodstream. The family member encoded by this gene is found in pancreatic islets and does not exhibit phosphohydrolase activity, but it is a major target of cell-mediated autoimmunity in diabetes. Several alternatively spliced transcript variants of this gene have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

G6PC2: May hydrolyze glucose-6-phosphate to glucose in the endoplasmic reticulum. May be responsible for glucose production through glycogenolysis and gluconeogenesis. Belongs to the glucose-6-phosphatase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; EC 3.1.3.9; Membrane protein, multi-pass; Phosphatase (non-protein); Carbohydrate Metabolism - glycolysis and gluconeogenesis; Transporter, SLC family; Carbohydrate Metabolism - galactose; Carbohydrate Metabolism - starch and sucrose; Endoplasmic reticulum; Transporter

Chromosomal Location of Human Ortholog: 2q24.3

Cellular Component: endoplasmic reticulum membrane; integral to membrane

Molecular Function: glucose-6-phosphatase activity

Biological Process: dephosphorylation; hexose transport; carbohydrate metabolic process; pathogenesis; glucose transport; glucose homeostasis; transmembrane transport; regulation of insulin secretion; gluconeogenesis

Disease: Fasting Plasma Glucose Level Quantitative Trait Locus 1