PTK2 sirna

PTK2 siRNA (Mouse)

Cat. Num. AAA8206966

• Gene Names: Ptk2; FAK; FRNK; Fadk; p125FAK; mKIAA4203
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PTK2; PTK2 siRNA (Mouse); FADK; FAK; FAK1; KIAA4203; Focal adhesion kinase 1; FADK 1; Focal adhesion kinase-related nonkinase; FRNK; Protein-tyrosine kinase 2; p125FAK; pp125FAK; PTK2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: PTK2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 360

• Applicable Applications for PTK2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse PTK2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PTK2 sirna

siRNA to inhibit PTK2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 194353974
• NCBI GeneID: 14083
• NCBI Accession #: NP_001123881.1
• NCBI GenBank Nucleotide #: NM_001130409.1
• UniProt Accession #: P34152; O08578; Q5DTH7; Q8C513; Q8CFH7; Q8CHM2; Q8K2S0; Q9DAW3
• Molecular Weight: 39,930 Da
• NCBI Official Full Name: focal adhesion kinase 1 isoform 2
• NCBI Official Synonym Full Names: PTK2 protein tyrosine kinase 2
• NCBI Official Symbol: Ptk2
• NCBI Official Synonym Symbols: FAK; FRNK; Fadk; p125FAK; mKIAA4203
• NCBI Protein Information: focal adhesion kinase 1
• UniProt Protein Name: Focal adhesion kinase 1
• Protein Family: Focal adhesion kinase
• UniProt Gene Name: Ptk2
• UniProt Synonym Gene Names: Fadk; Fak; Fak1; Kiaa4203; FADK 1; FRNK
• UniProt Entry Name: FAK1_MOUSE

Uniprot Description

FAK: a tyrosine kinase of the FAK family required for cell migration and contact-dependent survival signaling. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Downstream of integrins and Src, upstream of Ras/MAPK. Localizes to focal adhesions that form between cells growing in the presence of extracellular matrix constituents. Interacts with CAS family members and with GIT1, SORBS1 and BCAR3. Interacts with Shb. Required for full Ras transformation of fibroblasts. Increased expression in breast and other cancers, related to chromosome 8q amplification. Overexpression and activation associated with increased migration, invasion and progression of ovarian cancer, and with progression in hepatocellular carcinoma, thyroid cancer, and acute myelogenous leukemia. siRNA increases chemosensitivity of pancreatic adenocarcinoma xenografts. Inhibitor: ISI15421 (antisense). Four splice-variant isoforms have been observed.

Protein type: Kinase, protein; EC 2.7.10.2; Protein kinase, TK; Protein kinase, tyrosine (non-receptor); TK group; Fak family

Cellular Component: focal adhesion; basolateral plasma membrane; cytosol; nuclear body; extrinsic to internal side of plasma membrane; cytoskeleton; membrane; lamellipodium; apical plasma membrane; cytoplasm; stress fiber; plasma membrane; nucleolus; nucleus; cell junction; sarcolemma

Molecular Function: identical protein binding; nucleotide binding; non-membrane spanning protein tyrosine kinase activity; actin binding; protein kinase binding; protein kinase activity; integrin binding; transferase activity; JUN kinase binding; signal transducer activity; protein binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; protein complex binding; phosphoinositide 3-kinase binding; SH2 domain binding; kinase activity; ATP binding; receptor binding

Biological Process: extracellular matrix organization and biogenesis; positive regulation of cell adhesion; establishment of nucleus localization; peptidyl-tyrosine phosphorylation; multicellular organismal development; protein amino acid autophosphorylation; positive regulation of smooth muscle cell proliferation; neuron migration; negative regulation of synaptogenesis; positive regulation of smooth muscle cell migration; positive regulation of vasodilation; cellular chloride ion homeostasis; protein amino acid phosphorylation; positive regulation of synaptic transmission; regulation of cell shape; regulation of cell adhesion mediated by integrin; transforming growth factor beta receptor signaling pathway; positive regulation of phagocytosis; positive regulation of cell proliferation; ephrin receptor signaling pathway; JNK cascade; negative regulation of axonogenesis; angiogenesis; vasculogenesis; negative regulation of cell migration; regulation of cell adhesion; integrin-mediated signaling pathway; epidermal growth factor receptor signaling pathway; blood vessel development; central nervous system neuron axonogenesis; regulation of osteoblast differentiation; MAPKKK cascade; signal complex assembly; microtubule cytoskeleton organization and biogenesis; negative regulation of organ growth; positive regulation of cell growth; regulation of cell proliferation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; positive regulation of protein kinase activity; innate immune response; endothelial cell migration; positive regulation of protein amino acid phosphorylation; negative regulation of cell-cell adhesion; cell motility; phosphorylation; positive regulation of cell migration; negative regulation of apoptosis