AWAT2 sirna

AWAT2 siRNA (Human)

Cat. Num. AAA8206826

• Gene Names: AWAT2; WS; DC4; ARAT; MFAT; DGAT2L4
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: AWAT2; AWAT2 siRNA (Human); DC4; DGAT2L4; MFAT; WS; Acyl-CoA wax alcohol acyltransferase 2; Acyl-CoA retinol O-fatty-acyltransferase; ARAT; Retinol O-fatty-acyltransferase; Diacylglycerol O-acyltransferase 2-like protein 4; Diacylglycerol O-acyltransferase candidate 4; hDC4; Long-chain-alcohol O-fatty-acyltransferase 2; Multifunctional O-acyltransferase; Wax synthase; hWS; AWAT2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: AWAT2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 333

• Applicable Applications for AWAT2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human AWAT2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for AWAT2 sirna

siRNA to inhibit AWAT2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 51317389
• NCBI GeneID: 158835
• NCBI Accession #: NP_001002254.1
• NCBI GenBank Nucleotide #: NM_001002254.1
• UniProt Accession #: Q6E213; Q6IEE3; Q6P437
• Molecular Weight: 38,094 Da
• NCBI Official Full Name: acyl-CoA wax alcohol acyltransferase 2
• NCBI Official Synonym Full Names: acyl-CoA wax alcohol acyltransferase 2
• NCBI Official Symbol: AWAT2
• NCBI Official Synonym Symbols: WS; DC4; ARAT; MFAT; DGAT2L4
• NCBI Protein Information: acyl-CoA wax alcohol acyltransferase 2
• UniProt Protein Name: Acyl-CoA wax alcohol acyltransferase 2
• Protein Family: Acyl-CoA wax alcohol acyltransferase
• UniProt Gene Name: AWAT2
• UniProt Synonym Gene Names: DC4; DGAT2L4; MFAT; WS; ARAT; Retinol O-fatty-acyltransferase; hDC4; hWS
• UniProt Entry Name: AWAT2_HUMAN

NCBI Description

This gene encodes an enzyme belonging to the diacylglycerol acyltransferase family. This enzyme produces wax esters by the esterification of long chain (or wax) alcohols with acyl-CoA-derived fatty acids. It functions in lipid metabolism in the skin, mostly in undifferentiated peripheral sebocytes. This enzyme may also have acyl-CoA:retinol acyltransferase activities, where it catalyzes the synthesis of diacylglycerols and retinyl esters. [provided by RefSeq, May 2010]

Uniprot Description

DGAT2L4: Acyltransferase that predominantly esterify long chain (wax) alcohols with acyl-CoA-derived fatty acids to produce wax esters. Wax esters are enriched in sebum, suggesting that it plays a central role in lipid metabolism in skin. Has no activity using decyl alcohol and significantly prefers the C16 and C18 alcohols. May also have 2-acylglycerol O-acyltransferase (MGAT) and acyl- CoA:retinol acyltransferase (ARAT) activities, to catalyze the synthesis of diacylglycerols and retinyl esters; however this activity is unclear in vivo. Belongs to the diacylglycerol acyltransferase family.

Protein type: Cofactor and Vitamin Metabolism - retinol; Lipid Metabolism - glycerolipid; Transferase; Membrane protein, integral; EC 2.3.1.75; EC 2.3.1.76; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: Xq13.1

Cellular Component: endoplasmic reticulum membrane; integral to membrane

Molecular Function: retinol O-fatty-acyltransferase activity; long-chain-alcohol O-fatty-acyltransferase activity

Biological Process: retinol metabolic process; wax biosynthetic process