SMO sirna

SMO siRNA (Human)

Cat. Num. AAA8206425

• Gene Names: SMO; Gx; SMOH; FZD11
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SMO; SMO siRNA (Human); SMOH; Smoothened homolog; Protein Gx; SMO sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SMO siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 787

• Applicable Applications for SMO sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SMO gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SMO sirna

siRNA to inhibit SMO expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 5032099
• NCBI GeneID: 6608
• NCBI Accession #: NP_005622.1
• NCBI GenBank Nucleotide #: NM_005631.4
• UniProt Accession #: Q99835; A4D1K5
• Molecular Weight: 86,397 Da
• NCBI Official Full Name: smoothened homolog
• NCBI Official Synonym Full Names: smoothened, frizzled class receptor
• NCBI Official Symbol: SMO
• NCBI Official Synonym Symbols: Gx; SMOH; FZD11
• NCBI Protein Information: smoothened homolog
• UniProt Protein Name: Smoothened homolog
• Protein Family: Smoothelin
• UniProt Gene Name: SMO
• UniProt Synonym Gene Names: SMOH; SMO
• UniProt Entry Name: SMO_HUMAN

NCBI Description

The protein encoded by this gene is a G protein-coupled receptor that interacts with the patched protein, a receptor for hedgehog proteins. The encoded protein tranduces signals to other proteins after activation by a hedgehog protein/patched protein complex. [provided by RefSeq, Jul 2010]

Uniprot Description

SMO: G protein-coupled receptor that probably associates with the patched protein (PTCH) to transduce the hedgehog's proteins signal. Binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO). Required for the accumulation of KIF7 and GLI3 in the cilia. Belongs to the G-protein coupled receptor Fz/Smo family.

Protein type: Receptor, GPCR; GPCR, Fz/Smo family; Cell development/differentiation; Membrane protein, integral; Oncoprotein; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 7q32.3

Cellular Component: Golgi apparatus; intracellular membrane-bound organelle; integral to membrane; plasma membrane; caveola

Molecular Function: G-protein coupled receptor activity; Wnt-protein binding; protein binding; Wnt receptor activity; patched binding; drug binding

Biological Process: developmental growth; central nervous system neuron differentiation; ventral midline determination; negative regulation of epithelial cell differentiation; skeletal muscle fiber development; cell fate specification; thalamus development; negative regulation of transcription from RNA polymerase II promoter; Wnt receptor signaling pathway through beta-catenin; anterior/posterior pattern formation; positive regulation of mesenchymal cell proliferation; positive regulation of smoothened signaling pathway; heart looping; vasculogenesis; activation of hh target transcription factor; neural crest cell migration; dorsoventral neural tube patterning; positive regulation of neuroblast proliferation; smoothened signaling pathway; hair follicle morphogenesis; protein stabilization; smoothened signaling pathway in regulation of granule cell precursor cell proliferation; in utero embryonic development; multicellular organism growth; smoothened signaling pathway in ventral spinal cord patterning; forebrain morphogenesis; negative regulation of DNA binding; negative regulation of hair follicle development; odontogenesis of dentine-containing teeth; osteoblast differentiation; G-protein coupled receptor protein signaling pathway; positive regulation of protein import into nucleus; cerebellar cortex morphogenesis; myoblast migration; midgut development; positive regulation of transcription from RNA polymerase II promoter; positive regulation of epithelial cell proliferation; negative regulation of apoptosis