POLD1 sirna

POLD1 siRNA (Human)

Cat. Num. AAA8205595

• Gene Names: POLD1; CDC2; MDPL; POLD; CRCS10
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: POLD1; POLD1 siRNA (Human); POLD; DNA polymerase delta catalytic subunit; DNA polymerase subunit delta p125; POLD1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: POLD1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1107

• Applicable Applications for POLD1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human POLD1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for POLD1 sirna

siRNA to inhibit POLD1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 379030588
• NCBI GeneID: 5424
• NCBI Accession #: NP_001243778.1
• NCBI GenBank Nucleotide #: NM_001256849.1
• UniProt Accession #: P28340; Q8NER3; Q96H98
• Molecular Weight: 123,631 Da
• NCBI Official Full Name: DNA polymerase delta catalytic subunit 1
• NCBI Official Synonym Full Names: polymerase (DNA directed), delta 1, catalytic subunit
• NCBI Official Symbol: POLD1
• NCBI Official Synonym Symbols: CDC2; MDPL; POLD; CRCS10
• NCBI Protein Information: DNA polymerase delta catalytic subunit
• UniProt Protein Name: DNA polymerase delta catalytic subunit
• Protein Family: DNA polymerase
• UniProt Gene Name: POLD1
• UniProt Synonym Gene Names: POLD
• UniProt Entry Name: DPOD1_HUMAN

NCBI Description

This gene encodes the 125-kDa catalytic subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 6. [provided by RefSeq, Mar 2012]

Uniprot Description

POLD1: Possesses two enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Required with its accessory proteins (proliferating cell nuclear antigen (PCNA) and replication factor C (RFC) or activator 1) for leading strand synthesis. Also involved in completing Okazaki fragments initiated by the DNA polymerase alpha/primase complex. Belongs to the DNA polymerase type-B family.

Protein type: Hydrolase; Nucleotide Metabolism - pyrimidine; Nucleotide Metabolism - purine; DNA replication; EC 2.7.7.7; DNA repair, damage; Transferase

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: delta DNA polymerase complex; nucleoplasm; nucleotide-excision repair complex; membrane; cytoplasm; nucleus

Molecular Function: protein binding; DNA binding; metal ion binding; 4 iron, 4 sulfur cluster binding; DNA-directed DNA polymerase activity; 3'-5'-exodeoxyribonuclease activity; nucleotide binding; chromatin binding

Biological Process: mismatch repair; DNA strand elongation during DNA replication; DNA repair; telomere maintenance via semi-conservative replication; base-excision repair, gap-filling; DNA synthesis during DNA repair; DNA replication proofreading; base-excision repair; nucleotide-excision repair; transcription-coupled nucleotide-excision repair; fatty acid homeostasis; telomere maintenance via recombination; mitotic cell cycle; nucleotide-excision repair, DNA gap filling; DNA catabolic process, exonucleolytic; DNA replication; telomere maintenance; response to UV

Disease: Colorectal Cancer, Susceptibility To, 10; Mandibular Hypoplasia, Deafness, Progeroid Features, And Lipodystrophy Syndrome