COL4A3BP sirna

COL4A3BP siRNA (Mouse)

Cat. Num. AAA8205375

• Gene Names: Col4a3bp; CERT; GPBP; AU016711; 2810404O15Rik; 9230101K08Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: COL4A3BP; COL4A3BP siRNA (Mouse); STARD11; Collagen type IV alpha-3-binding protein; Ceramide transfer protein; Goodpasture antigen-binding protein; GPBP; START domain-containing protein 11; StARD11; StAR-related lipid transfer protein 11; COL4A3BP sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: COL4A3BP siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 598

• Applicable Applications for COL4A3BP sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse COL4A3BP gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for COL4A3BP sirna

siRNA to inhibit COL4A3BP expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 255982524
• NCBI GeneID: 68018
• NCBI Accession #: NP_001157694.1
• NCBI GenBank Nucleotide #: NM_001164222.1
• UniProt Accession #: Q9EQG9; Q3TQF6; Q8BNN8; Q8C8H3; Q8CAR3; Q91WB1; Q9CU52; Q9EQG8
• Molecular Weight: 35,929 Da
• NCBI Official Full Name: collagen type IV alpha-3-binding protein isoform 2
• NCBI Official Synonym Full Names: collagen, type IV, alpha 3 (Goodpasture antigen) binding protein
• NCBI Official Symbol: Col4a3bp
• NCBI Official Synonym Symbols: CERT; GPBP; AU016711; 2810404O15Rik; 9230101K08Rik
• NCBI Protein Information: collagen type IV alpha-3-binding protein
• UniProt Protein Name: Collagen type IV alpha-3-binding protein
• Protein Family: Collagen type IV alpha-3-binding protein
• UniProt Gene Name: Col4a3bp
• UniProt Synonym Gene Names: Stard11; GPBP; StARD11
• UniProt Entry Name: C43BP_MOUSE

Uniprot Description

COL4A3BP: a protein with a lipid-binding domain that mediates intracellular trafficking of ceramide in a non-vesicular manner. Implicated in human autoimmune diseases via its association with the Goodpasture antigen. Phosphorylation on Ser-132 decreases the affinity toward phosphatidylinositol 4-phosphate at Golgi membranes and reduces ceramide transfer activity. GPBP has been reported to have Ser/Thr protein kinase activity towards the Goodpasture (GP) antigen, but lacks a conventional serine/threonine kinase domain. Two human isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.9; Kinase, protein; Protein kinase, atypical; Lipid-binding; ATYPICAL group; COL4A3BP family

Cellular Component: nucleoplasm; Golgi apparatus; mitochondrion; perinuclear region of cytoplasm; endoplasmic reticulum; cytoplasm; cytosol

Molecular Function: kinase activity; lipid binding

Biological Process: endoplasmic reticulum organization and biogenesis; heart morphogenesis; cell proliferation; muscle contraction; transport; in utero embryonic development; cell morphogenesis; lipid homeostasis; lipid transport; ceramide metabolic process; signal transduction; phosphorylation