GRHL2 sirna

GRHL2 siRNA (Mouse)

Cat. Num. AAA8205310

• Gene Names: Grhl2; BOM; Tcfcp2l3; 0610015A08Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: GRHL2; GRHL2 siRNA (Mouse); BOM; TCFCP2L3; Grainyhead-like protein 2 homolog; Brother of mammalian grainyhead; Transcription factor CP2-like 3; GRHL2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: GRHL2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 625

• Applicable Applications for GRHL2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse GRHL2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for GRHL2 sirna

siRNA to inhibit GRHL2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 46810275
• NCBI GeneID: 252973
• NCBI Accession #: NP_080772.2
• NCBI GenBank Nucleotide #: NM_026496.4
• UniProt Accession #: Q8K5C0; Q80UZ5
• Molecular Weight: 71,195 Da
• NCBI Official Full Name: grainyhead-like protein 2 homolog
• NCBI Official Synonym Full Names: grainyhead-like 2 (Drosophila)
• NCBI Official Symbol: Grhl2
• NCBI Official Synonym Symbols: BOM; Tcfcp2l3; 0610015A08Rik
• NCBI Protein Information: grainyhead-like protein 2 homolog
• UniProt Protein Name: Grainyhead-like protein 2 homolog
• Protein Family: Grainyhead-like protein
• UniProt Gene Name: Grhl2
• UniProt Synonym Gene Names: Bom; Tcfcp2l3
• UniProt Entry Name: GRHL2_MOUSE

Uniprot Description

GRHL2: May function as a transcription factor. Defects in GRHL2 are the cause of deafness autosomal dominant type 28 (DFNA28). DFNA28 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. DFNA28 is characterized by mild to moderate hearing loss across most frequencies that progressed to severe loss in the higher frequencies by the fifth decade. Age at onset varied, with the earliest case documented at 7 years of age. Belongs to the grh/CP2 family. Grainyhead subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor

Cellular Component: membrane; intercellular junction; nucleus

Molecular Function: DNA binding; chromatin DNA binding; sequence-specific DNA binding; transcription factor activity

Biological Process: neural tube development; epidermis development; camera-type eye development; transcription, DNA-dependent; in utero embryonic development; multicellular organism growth; respiratory tube development; negative regulation of keratinocyte differentiation; embryonic cranial skeleton morphogenesis; positive regulation of telomerase activity; regulation of transcription from RNA polymerase II promoter; cell proliferation; regulation of transcription, DNA-dependent; regulation of gene expression; neural tube closure; embryonic organ development; positive regulation of transcription from RNA polymerase II promoter; embryonic digit morphogenesis; brain development; cell adhesion