GABARAPL2 sirna

GABARAPL2 siRNA (Mouse)

Cat. Num. AAA8205272

• Gene Names: Gabarapl2; Gef2; GATE-16; AI173605; 0610012F20Rik; 2900019O08Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: GABARAPL2; GABARAPL2 siRNA (Mouse); Gamma-aminobutyric acid receptor-associated protein-like 2; GABA(A) receptor-associated protein-like 2; Golgi-associated ATPase enhancer of 16 kDa; GATE-16; GABARAPL2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: GABARAPL2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 117

• Applicable Applications for GABARAPL2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse GABARAPL2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for GABARAPL2 sirna

siRNA to inhibit GABARAPL2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 31542873
• NCBI GeneID: 93739
• NCBI Accession #: NP_080969.2
• NCBI GenBank Nucleotide #: NM_026693.5
• UniProt Accession #: P60521; O08765; Q9DCP8; Q9UQF7
• Molecular Weight: 13,667 Da
• NCBI Official Full Name: gamma-aminobutyric acid receptor-associated protein-like 2
• NCBI Official Synonym Full Names: gamma-aminobutyric acid (GABA) A receptor-associated protein-like 2
• NCBI Official Symbol: Gabarapl2
• NCBI Official Synonym Symbols: Gef2; GATE-16; AI173605; 0610012F20Rik; 2900019O08Rik
• NCBI Protein Information: gamma-aminobutyric acid receptor-associated protein-like 2
• UniProt Protein Name: Gamma-aminobutyric acid receptor-associated protein-like 2
• Protein Family: Gamma-aminobutyric acid receptor-associated protein
• UniProt Gene Name: Gabarapl2
• UniProt Synonym Gene Names: GATE-16
• UniProt Entry Name: GBRL2_MOUSE

Uniprot Description

GABARAPL2: is a ubiquitin-like protein that is a constituent of the ATG8-conjugation system, one of two evolutionarily conserved phosphatidylethanolamine conjugation systems necessary for the formation of the autophagosome. The human ATG8 system includes seven ubiquitin-like light chain proteins (LCPs) that are homologs of yeast LC3: MAP1LC3A, -B, -C, GABARAP, GABARAPL1, -2, and -3. Pro-LCPs are cleaved by ATG4B to expose a C-terminal glycine residue, the cytosolic LCP-I form. The exposed C-terminus is conjugated to the head group amine of phosphatidylethanolamine (PE) through an amide bond by a sequence of ubiquitination-like reactions that involves an E1 (ATG7), an E2 (ATG3), and an E3 (a complex including ATG5, ATG12, and ATG16L). The PE-congugated form (LCP-II) is tightly associated with the autophagosomal membrane. The LCP-II forms can also be delipidated by the ATG4 proteases: most of the LCPs are delipidated and liberated from the membrane before autophagosomes fuse with lysosomes. Implicated in intra-Golgi transport and post-mitotic Golgi re-assembly. Modulates the activity of SNAREs in the Golgi apparatus and is therefore an essential component of intra-Golgi transport and post-mitotic Golgi re-assembly. It first stimulates the ATPase activity of NSF, which in turn stimulates the association with GOSR1. Interacts with GABRG2, NSF, GOSR1 and beta-tubulin. Interacts with ULK1. Phosphorylated upon DNA damage, probably by ATM or ATR.

Protein type: Microtubule-binding; Ubiquitin-like modifier; Vesicle; Autophagy; Adaptor/scaffold

Cellular Component: Golgi membrane; Golgi apparatus; extrinsic to membrane; cell; cytoplasm; autophagic vacuole; cytoplasmic vesicle; intracellular; cytosol

Molecular Function: protein binding; microtubule binding

Biological Process: intra-Golgi vesicle-mediated transport; protein transport; transport; mitochondrion degradation; autophagy; cellular response to nitrogen starvation; autophagic vacuole formation