Rabbit anti-Human RAF Polyclonal Antibody | anti-RAF antibody

RAF (Serine/Threonine-protein Kinase A-Raf, Proto-oncogene A-Raf-1, Proto-oncogene A-Raf, Proto-oncogene Pks, ARAF, ARAF1, PKS, PKS2) (FITC)

Cat. Num. AAA6391905

• Gene Names: ARAF; PKS2; A-RAF; ARAF1; RAFA1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RAF; Polyclonal Antibody; RAF (Serine/Threonine-protein Kinase A-Raf; Proto-oncogene A-Raf-1; Proto-oncogene A-Raf; Proto-oncogene Pks; ARAF; ARAF1; PKS; PKS2) (FITC); anti-RAF antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ARAF.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Sequence Length: 2466

• Applicable Applications for anti-RAF antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human ARAF, aa1-606 (NP_001645.1).
• Immunogen Sequence: MEPPRGPPANGAEPSRAVGTVKVYLPNKQRTVVTVRDGMSVYDSLDKALKVRGLNQDCCVVYRLIKGRKTVTAWDTAIAPLDGEELIVEVLEDVPLTMHNFVRKTFFSLAFCDFCLKFLFHGFRCQTCGYKFHQHCSSKVPTVCVDMSTNRQQFYHSVQDLSGGSRQHEAPSNRPLNELLTPQGPSPRTQHCDPEHFPFPAPANAPLQRIRSTSTPNVHMVSTTAPMDSNLIQLTGQSFSTDAAGSRGGSDGTPRGSPSPASVSSGRKSPHSKSPAEQRERKSLADDKKKVKNLGYRDSGYYWEVPPSEVQLLKRIGTGSFGTVFRGRWHGDVAVKVLKVSQPTAEQAQAFKNEMQVLRKTRHVNILLFMGFMTRPGFAIITQWCEGSSLYHHLHVADTRFDMVQLIDVARQTAQGMDYLHAKNIIHRDLKSNNIFLHEGLTVKIGDFGLATVKTRWSGAQPLEQPSGSVLWMAAEVIRMQDPNPYSFQSDVYAYGVVLYELMTGSLPYSHIGCRDQIIFMVGRGYLSPDLSKISSNCPKAMRRLLSDCLKFQREERPLFPQILATIELLQRSLPKIERSASEPSLHRTQADELPACLLSAARLVP
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ARAF rabbit polyclonal antibody. Western Blot analysis of ARAF expression in human placenta.)

Western Blot (WB)

(Western Blot analysis of ARAF expression in transfected 293T cell line by ARAF polyclonal antibody. Lane 1: ARAF transfected lysate (67.6kD). Lane 2: Non-transfected lysate.)

Testing Data

(Proximity Ligation Analysis (PLA) of protein-protein interactions between ARAF and BRAF. HeLa cells were stained with ARAF rabbit purified polyclonal 1:1200 and BRAF mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)

Related Product Information for anti-RAF antibody

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.

Product Categories/Family for anti-RAF antibody

Antibodies; Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 4502192
• NCBI GeneID: 369
• NCBI Accession #: NM_001654.1
• NCBI GenBank Nucleotide #: NM_001654.1
• NCBI Official Full Name: Homo sapiens v-raf murine sarcoma 3611 viral oncogene homolog (ARAF), mRNA
• NCBI Official Synonym Full Names: A-Raf proto-oncogene, serine/threonine kinase
• NCBI Official Symbol: ARAF
• NCBI Official Synonym Symbols: PKS2; A-RAF; ARAF1; RAFA1
• NCBI Protein Information: serine/threonine-protein kinase A-Raf
• Protein Family: Raftlin

NCBI Description

This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2012]