SLC1A2 sirna

SLC1A2 siRNA (Rat)

Cat. Num. AAA8205092

• Gene Names: Slc1a2; Glt; Eaat2; Glt-1
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SLC1A2; SLC1A2 siRNA (Rat); EAAT2; GLT1; Excitatory amino acid transporter 2; GLT-1; Sodium-dependent glutamate/aspartate transporter 2; GLUT-R; Solute carrier family 1 member 2; SLC1A2 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: SLC1A2 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 570

• Applicable Applications for SLC1A2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat SLC1A2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SLC1A2 sirna

siRNA to inhibit SLC1A2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 690969210
• NCBI GeneID: 29482
• NCBI Accession #: NP_001289018.1
• NCBI GenBank Nucleotide #: NM_001302089.1
• UniProt Accession #: P31596
• Molecular Weight: 61,847 Da
• NCBI Official Full Name: excitatory amino acid transporter 2 isoform c
• NCBI Official Synonym Full Names: solute carrier family 1 (glial high affinity glutamate transporter), member 2
• NCBI Official Symbol: Slc1a2
• NCBI Official Synonym Symbols: Glt; Eaat2; Glt-1
• NCBI Protein Information: excitatory amino acid transporter 2
• UniProt Protein Name: Excitatory amino acid transporter 2
• Protein Family: Excitatory amino acid transporter
• UniProt Gene Name: Slc1a2
• UniProt Synonym Gene Names: Eaat2; Glt1; GLUT-R
• UniProt Entry Name: EAA2_RAT

NCBI Description

mediates dihydrokainate-sensitive glutamate transport; may play a role in presynaptic terminal glutamate transport and synaptic transmission [RGD, Feb 2006]

Uniprot Description

GLT1: an integral membrane protein belonging to the sodium:dicarboxylate (SDF) symporter family. Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by co-transporting sodium.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Transporter, SLC family; Transporter

Cellular Component: presynaptic membrane; neuron projection; axon; plasma membrane; dendritic spine; integral to membrane; synapse; dendritic shaft; axolemma

Molecular Function: high-affinity glutamate transmembrane transporter activity; glutamate:sodium symporter activity; sodium:dicarboxylate symporter activity; L-glutamate transmembrane transporter activity

Biological Process: response to drug; nervous system development; response to light stimulus; L-glutamate transport; multicellular organism growth; multicellular organismal aging; response to amino acid stimulus; visual behavior; L-glutamate import; positive regulation of glucose import; telencephalon development; neurotransmitter transport; adult behavior; cellular response to extracellular stimulus; response to wounding