NOD1 sirna

NOD1 siRNA (Mouse)

Cat. Num. AAA8204450

• Gene Names: Nod1; Card4; Nlrc1; C230079P11; F830007N14Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: NOD1; NOD1 siRNA (Mouse); CARD4; Nucleotide-binding oligomerization domain-containing protein 1; Caspase recruitment domain-containing protein 4; NOD1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: NOD1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 953

• Applicable Applications for NOD1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse NOD1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for NOD1 sirna

siRNA to inhibit NOD1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 283806725
• NCBI GeneID: 107607
• NCBI Accession #: NP_001164478.1
• NCBI GenBank Nucleotide #: NM_001171007.1
• UniProt Accession #: Q8BHB0; Q8BUT6
• Molecular Weight: 107,740 Da
• NCBI Official Full Name: nucleotide-binding oligomerization domain-containing protein 1
• NCBI Official Synonym Full Names: nucleotide-binding oligomerization domain containing 1
• NCBI Official Symbol: Nod1
• NCBI Official Synonym Symbols: Card4; Nlrc1; C230079P11; F830007N14Rik
• NCBI Protein Information: nucleotide-binding oligomerization domain-containing protein 1
• UniProt Protein Name: Nucleotide-binding oligomerization domain-containing protein 1
• Protein Family: Nucleotide-binding oligomerization domain-containing protein
• UniProt Gene Name: Nod1
• UniProt Synonym Gene Names: Card4
• UniProt Entry Name: NOD1_MOUSE

Uniprot Description

NOD1: Enhances caspase-9-mediated apoptosis. Induces NF-kappa- B activity via RIPK2 and IKK-gamma. Confers responsiveness to intracellular bacterial lipopolysaccharides (LPS). Forms an intracellular sensing system along with ARHGEF2 for the detection of microbial effectors during cell invasion by pathogens. Required for RHOA and RIPK2 dependent NF-kappa-B signaling pathways activation upon S.flexneri cell invasion. Involved not only in sensing peptidoglycan (PGN)-derived muropeptides but also in the activation of NF-kappa-B by Shigella effector proteins IpgB2 and OspB. Homodimer. Self-associates. Binds to caspase-9 and RIPK2 by CARD-CARD interaction. Interacts with ARHGEF2. Highly expressed in adult heart, skeletal muscle, pancreas, spleen and ovary. Also detected in placenta, lung, liver, kidney, thymus, testis, small intestine and colon.

Cellular Component: membrane; basolateral plasma membrane; cytoplasm; plasma membrane

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; nucleotide binding; CARD domain binding; ATP binding

Biological Process: caspase activation; positive regulation of I-kappaB kinase/NF-kappaB cascade; detection of bacterium; immune system process; apoptosis; positive regulation of dendritic cell antigen processing and presentation; positive regulation of interleukin-6 production; positive regulation of JNK cascade; positive regulation of caspase activity; defense response; positive regulation of tumor necrosis factor production; positive regulation of stress-activated MAPK cascade; activation of NF-kappaB transcription factor; regulation of apoptosis; interleukin-8 biosynthetic process; defense response to Gram-positive bacterium; defense response to bacterium; innate immune response